Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis
Само за регистроване кориснике
2010
Аутори
Brajović, GavriloStefanović, Gordana
Ilić, Vesna
Petrović, Sonja
Stefanović, Neda
Nikolić-Jakoba, Nataša
Milošević-Jovčić, Nadežda
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which c...ontained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.
Кључне речи:
Fibronectin / glycosylation / immunoglobulin G / periodontitisИзвор:
Journal of Periodontology, 2010, 81, 10, 1472-1480Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Матичне ћелије зубне пулпе - молекулске и функционалне карактеристике и значај у регенерацији ткива зуба (RS-MESTD-MPN2006-2010-156017)
DOI: 10.1902/jop.2010.100053
ISSN: 0022-3492
PubMed: 20450403
WoS: 000283023200015
Scopus: 2-s2.0-78249289636
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Brajović, Gavrilo AU - Stefanović, Gordana AU - Ilić, Vesna AU - Petrović, Sonja AU - Stefanović, Neda AU - Nikolić-Jakoba, Nataša AU - Milošević-Jovčić, Nadežda PY - 2010 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1563 AB - Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480. PB - Wiley, Hoboken T2 - Journal of Periodontology T1 - Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis VL - 81 IS - 10 SP - 1472 EP - 1480 DO - 10.1902/jop.2010.100053 ER -
@article{ author = "Brajović, Gavrilo and Stefanović, Gordana and Ilić, Vesna and Petrović, Sonja and Stefanović, Neda and Nikolić-Jakoba, Nataša and Milošević-Jovčić, Nadežda", year = "2010", abstract = "Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.", publisher = "Wiley, Hoboken", journal = "Journal of Periodontology", title = "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis", volume = "81", number = "10", pages = "1472-1480", doi = "10.1902/jop.2010.100053" }
Brajović, G., Stefanović, G., Ilić, V., Petrović, S., Stefanović, N., Nikolić-Jakoba, N.,& Milošević-Jovčić, N.. (2010). Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology Wiley, Hoboken., 81(10), 1472-1480. https://doi.org/10.1902/jop.2010.100053
Brajović G, Stefanović G, Ilić V, Petrović S, Stefanović N, Nikolić-Jakoba N, Milošević-Jovčić N. Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology. 2010;81(10):1472-1480. doi:10.1902/jop.2010.100053 .
Brajović, Gavrilo, Stefanović, Gordana, Ilić, Vesna, Petrović, Sonja, Stefanović, Neda, Nikolić-Jakoba, Nataša, Milošević-Jovčić, Nadežda, "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis" in Journal of Periodontology, 81, no. 10 (2010):1472-1480, https://doi.org/10.1902/jop.2010.100053 . .