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dc.creatorShin, Ki-Hyuk
dc.creatorKim, Reuben H.
dc.creatorYu, Bo
dc.creatorKang, Mo K.
dc.creatorElashoff, David
dc.creatorChristensen, Russell
dc.creatorPucar, Ana
dc.creatorPark, No-Hee
dc.date.accessioned2020-07-02T12:31:49Z
dc.date.available2020-07-02T12:31:49Z
dc.date.issued2011
dc.identifier.issn1368-8375
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/1607
dc.description.abstractWe previously reported that wild type (wt) hnRNP G exhibited tumor suppressive activity in human oral squamous cell carcinoma (HOSCC) cell lines lacking hnRNP G. Wt hnRNP G markedly inhibited the proliferation capacity, anchorage independency and in vivo tumorigenicity of HOSCC cells and notably enhanced the DNA repair capabilities of these cells. In the present study, we studied the genetic and expression states of hnRNP G in normal, premalignant and malignant human oral tissues to further understand the relationship between the hnRNP G alterations and the development of human oral cancer. To correlate the cancer development and the level of hnRNP G expression, we performed an immunohistochemistry staining of hnRNP G in normal, premalignant and malignant human oral tissues. Moreover, we examined the entire coding regions of hnRNP G from selected samples to understand the cause of the alterations of the gene expression. The expression of hnRNP G was notably decreased or completely abolished in 80% of premalignant-dysplastic and malignant oral epithelial tissues, whereas 100% of normal and 90% of hyperplastic non-dysplastic epithelium showed high level of hnRNP G in the nucleus of the basal cell layers. Approximately 80% of HOSCC lacking the expression of hnRNP G showed genetic alteration in hnRNP G, i.e., point mutation and exonic deletion. This study suggest that genetic alterations and aberrant expression of hnRNP G occurring during oral carcinogenesis might be useful markers for the early detection of human oral cancer. Published by Elsevier Ltd.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationNational Institute of Dental and Craniofacial ResearchUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [DE14147, DE15902, DE17121, DE18295]
dc.rightsrestrictedAccess
dc.sourceOral Oncology
dc.subjecthnRNP Gen
dc.subjectOral canceren
dc.subjectImmunohistochemistryen
dc.subjectGenetic alterationen
dc.titleExpression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral canceren
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСхин, Ки-Хyук; Ким, Реубен Х.; Цхристенсен, Русселл; Канг, Мо К.; Еласхофф, Давид; Парк, Но-Хее; Yу, Бо; Пуцар, Aна;
dc.citation.volume47
dc.citation.issue11
dc.citation.spage1011
dc.citation.epage1016
dc.citation.other47(11): 1011-1016
dc.citation.rankaM21
dc.identifier.wos000296577500002
dc.identifier.doi10.1016/j.oraloncology.2011.07.012
dc.identifier.pmid21840245
dc.identifier.scopus2-s2.0-81155160938
dc.identifier.rcubconv_2960
dc.type.versionpublishedVersion


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