Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNA...s) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.
National Institute of Dental Research/National Institute of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 DE017170]
TY - JOUR
AU - Brinkmann, Ole
AU - Kastratović, Dragana A.
AU - Dimitrijević, Milovan
AU - Konstantinović, Vitomir
AU - Jelovac, Drago
AU - Antić, Jadranka
AU - Nesić, Vladimir S.
AU - Marković, Srđan Z.
AU - Martinović, Željko
AU - Akin, David
AU - Spielmann, Nadine
AU - Zhou, Hui
AU - Wong, David T.
PY - 2011
UR - http://smile.stomf.bg.ac.rs/handle/123456789/1608
AB - Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.
PB - Elsevier Science Bv, Amsterdam
T2 - Oral Oncology
T1 - Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population
VL - 47
IS - 1
SP - 51
EP - 55
DO - 10.1016/j.oraloncology.2010.10.009
ER -
@article{
author = "Brinkmann, Ole and Kastratović, Dragana A. and Dimitrijević, Milovan and Konstantinović, Vitomir and Jelovac, Drago and Antić, Jadranka and Nesić, Vladimir S. and Marković, Srđan Z. and Martinović, Željko and Akin, David and Spielmann, Nadine and Zhou, Hui and Wong, David T.",
year = "2011",
url = "http://smile.stomf.bg.ac.rs/handle/123456789/1608",
abstract = "Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Oral Oncology",
title = "Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population",
volume = "47",
number = "1",
pages = "51-55",
doi = "10.1016/j.oraloncology.2010.10.009"
}
Brinkmann, O., Kastratović, D. A., Dimitrijević, M., Konstantinović, V., Jelovac, D., Antić, J., Nesić, V. S., Marković, S. Z., Martinović, Ž., Akin, D., Spielmann, N., Zhou, H.,& Wong, D. T. (2011). Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population.
Oral Oncology
Elsevier Science Bv, Amsterdam., 47(1), 51-55.
https://doi.org/10.1016/j.oraloncology.2010.10.009
Brinkmann O, Kastratović DA, Dimitrijević M, Konstantinović V, Jelovac D, Antić J, Nesić VS, Marković SZ, Martinović Ž, Akin D, Spielmann N, Zhou H, Wong DT. Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population. Oral Oncology. 2011;47(1):51-55
Brinkmann Ole, Kastratović Dragana A., Dimitrijević Milovan, Konstantinović Vitomir, Jelovac Drago, Antić Jadranka, Nesić Vladimir S., Marković Srđan Z., Martinović Željko, Akin David, Spielmann Nadine, Zhou Hui, Wong David T., "Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population" Oral Oncology, 47, no. 1 (2011):51-55,
https://doi.org/10.1016/j.oraloncology.2010.10.009 .
