Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population

2011
Authors
Brinkmann, OleKastratović, Dragana A.
Dimitrijević, Milovan
Konstantinović, Vitomir

Jelovac, Drago

Antić, Jadranka

Nesić, Vladimir S.
Marković, Srđan Z.
Martinović, Željko
Akin, David
Spielmann, Nadine
Zhou, Hui
Wong, David T.
Article (Published version)

Metadata
Show full item recordAbstract
Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNA...s) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.
Keywords:
Oral cancer / Biomarkers / Salivary diagnostics / Proteome / TranscriptomeSource:
Oral Oncology, 2011, 47, 1, 51-55Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- National Institute of Dental Research/National Institute of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 DE017170]
DOI: 10.1016/j.oraloncology.2010.10.009
ISSN: 1368-8375
PubMed: 21109482
WoS: 000286872500010
Scopus: 2-s2.0-79551513238
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Institution/Community
Stomatološki fakultetTY - JOUR AU - Brinkmann, Ole AU - Kastratović, Dragana A. AU - Dimitrijević, Milovan AU - Konstantinović, Vitomir AU - Jelovac, Drago AU - Antić, Jadranka AU - Nesić, Vladimir S. AU - Marković, Srđan Z. AU - Martinović, Željko AU - Akin, David AU - Spielmann, Nadine AU - Zhou, Hui AU - Wong, David T. PY - 2011 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1608 AB - Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection. PB - Elsevier Science Bv, Amsterdam T2 - Oral Oncology T1 - Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population VL - 47 IS - 1 SP - 51 EP - 55 DO - 10.1016/j.oraloncology.2010.10.009 ER -
@article{ author = "Brinkmann, Ole and Kastratović, Dragana A. and Dimitrijević, Milovan and Konstantinović, Vitomir and Jelovac, Drago and Antić, Jadranka and Nesić, Vladimir S. and Marković, Srđan Z. and Martinović, Željko and Akin, David and Spielmann, Nadine and Zhou, Hui and Wong, David T.", year = "2011", abstract = "Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p lt 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Oral Oncology", title = "Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population", volume = "47", number = "1", pages = "51-55", doi = "10.1016/j.oraloncology.2010.10.009" }
Brinkmann, O., Kastratović, D. A., Dimitrijević, M., Konstantinović, V., Jelovac, D., Antić, J., Nesić, V. S., Marković, S. Z., Martinović, Ž., Akin, D., Spielmann, N., Zhou, H.,& Wong, D. T.. (2011). Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population. in Oral Oncology Elsevier Science Bv, Amsterdam., 47(1), 51-55. https://doi.org/10.1016/j.oraloncology.2010.10.009
Brinkmann O, Kastratović DA, Dimitrijević M, Konstantinović V, Jelovac D, Antić J, Nesić VS, Marković SZ, Martinović Ž, Akin D, Spielmann N, Zhou H, Wong DT. Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population. in Oral Oncology. 2011;47(1):51-55. doi:10.1016/j.oraloncology.2010.10.009 .
Brinkmann, Ole, Kastratović, Dragana A., Dimitrijević, Milovan, Konstantinović, Vitomir, Jelovac, Drago, Antić, Jadranka, Nesić, Vladimir S., Marković, Srđan Z., Martinović, Željko, Akin, David, Spielmann, Nadine, Zhou, Hui, Wong, David T., "Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population" in Oral Oncology, 47, no. 1 (2011):51-55, https://doi.org/10.1016/j.oraloncology.2010.10.009 . .