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dc.creatorRoganović, Jelena
dc.creatorRadenković, Miroslav
dc.creatorTanić, Nikola
dc.creatorTanić, Nasta
dc.creatorPetrović, Nina
dc.creatorStojić, Dragica
dc.date.accessioned2020-07-02T12:32:59Z
dc.date.available2020-07-02T12:32:59Z
dc.date.issued2011
dc.identifier.issn0909-8836
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/1625
dc.description.abstractThe aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M-3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N-G-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M-3 receptor level.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175021/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Oral Sciences
dc.subjectacetylcholineen
dc.subjectdiabetesen
dc.subjectrabbit parotid arteryen
dc.titleImpairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbiten
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСтојић, Драгица; Петровић, Нина; Рогановић, Јелена; Танић, Наста; Танић, Никола; Раденковић, Мирослав;
dc.citation.volume119
dc.citation.issue5
dc.citation.spage352
dc.citation.epage360
dc.citation.other119(5): 352-360
dc.citation.rankM21
dc.identifier.wos000294808000004
dc.identifier.doi10.1111/j.1600-0722.2011.00851.x
dc.identifier.pmid21896051
dc.identifier.scopus2-s2.0-80052538432
dc.type.versionpublishedVersion


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