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Nucleation of biomimetic hydroxyapatite

Nukleacija biomimetskog hidroksiapatita

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2011
1628.pdf (1.099Mb)
Authors
Čolović, Božana
Marković, Dejan
Jokanović, Vukoman
Article (Published version)
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Abstract
Introduction. The aim of the study was to assess the formation of biomimetic calcium hydroxyapatite (HAP) on the surface of different substrates. Material and Methods. Silica coated stainless steel tapes and thin polymer films (alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited on hydroxyapatite scaffold were used as substrate. Supersaturated simulated body fluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle's Minimum Essential Medium (EMEM) were used as bioactive liquid medium where biomimetic nucleation of HAP occurred. Infrared spectroscopy with Fourier transformation was used to analyze the formed phases, while scanning electron microscopy indicated the morphology of nucleated phase. Results. The results of measuring the mass with volume adjustments done by the BET method showed that the thickness of the film of nucleated calcium hydroxyapatite depended on the time that samples spent soaked in SBF-in as well as the type of selected biomimetic medium. Con...clusion. Biomimetic calcium hydroxyapatite is possible to produce by self nucleation on different substrates in the presence of simulating body fluid.

Uvod. Cilj ovog rada je bio da se ispita formiranje biomimetskog kalcijum-hidroksiapatita na površinama različitih supstrata. Materijal i metode rada. Kao supstrati su korišćeni tanki filmovi silicijum-dioksida naneti na čelične trake i tanki polimerni filmovi (alginat, celuloza, polilaktidkoglikolid) naneti na visokoporozni hidroksiapatitni nosač. Simulirana telesna tečnost (engl. simulated body fluid-SBF), kao i SBF kombinovan sa serumom iz fetusa govečeta ili Iglovim medijumom korišćeni su kao bioaktivni tečni medijum u kojem se odvija biomimetska nukleacija apatita. Za analizu formiranih faza korišćena je infracrvena spektroskopija sa Furijeovom transformacijom, dok je skening elektronska mikroskopija ukazivala na morfologiju nukleirane faze. Rezultati. Merenje mase uz korekciju zapremine primenom metode BET pokazalo je da debljina filma nukleiranog kalcijum-hidroksiapatita zavisi od vremena držanja uzoraka u SBF, kao i od vrste izabranog biomimetskog medijuma. Zaključak. Dobijanje... biomimetskog kalcijum-hidroksiapatita moguće je samonukleacijom na različitim supstratima i primenom SBF.

Keywords:
biomimetic method / bioactive thin films / biomimetic hydroxyapatite / nanostructure design / biomimetska metoda / bioaktivni tanki filmovi / biomimetski hidroksiapatit / dizajniranje nanostrukture
Source:
Stomatološki glasnik Srbije, 2011, 58, 1, 7-15
Publisher:
  • Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
Funding / projects:
  • Chemical and structural designing of nanomaterials for application in medicine and tissue engineering (RS-172026)

DOI: 10.2298/SGS1101007C

ISSN: 0039-1743

[ Google Scholar ]
URI
https://smile.stomf.bg.ac.rs/handle/123456789/1633
Collections
  • Radovi istraživača
Institution/Community
Stomatološki fakultet
TY  - JOUR
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Jokanović, Vukoman
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1633
AB  - Introduction. The aim of the study was to assess the formation of biomimetic calcium hydroxyapatite (HAP) on the surface of different substrates. Material and Methods. Silica coated stainless steel tapes and thin polymer films (alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited on hydroxyapatite scaffold were used as substrate. Supersaturated simulated body fluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle's Minimum Essential Medium (EMEM) were used as bioactive liquid medium where biomimetic nucleation of HAP occurred. Infrared spectroscopy with Fourier transformation was used to analyze the formed phases, while scanning electron microscopy indicated the morphology of nucleated phase. Results. The results of measuring the mass with volume adjustments done by the BET method showed that the thickness of the film of nucleated calcium hydroxyapatite depended on the time that samples spent soaked in SBF-in as well as the type of selected biomimetic medium. Conclusion. Biomimetic calcium hydroxyapatite is possible to produce by self nucleation on different substrates in the presence of simulating body fluid.
AB  - Uvod. Cilj ovog rada je bio da se ispita formiranje biomimetskog kalcijum-hidroksiapatita na površinama različitih supstrata. Materijal i metode rada. Kao supstrati su korišćeni tanki filmovi silicijum-dioksida naneti na čelične trake i tanki polimerni filmovi (alginat, celuloza, polilaktidkoglikolid) naneti na visokoporozni hidroksiapatitni nosač. Simulirana telesna tečnost (engl. simulated body fluid-SBF), kao i SBF kombinovan sa serumom iz fetusa govečeta ili Iglovim medijumom korišćeni su kao bioaktivni tečni medijum u kojem se odvija biomimetska nukleacija apatita. Za analizu formiranih faza korišćena je infracrvena spektroskopija sa Furijeovom transformacijom, dok je skening elektronska mikroskopija ukazivala na morfologiju nukleirane faze. Rezultati. Merenje mase uz korekciju zapremine primenom metode BET pokazalo je da debljina filma nukleiranog kalcijum-hidroksiapatita zavisi od vremena držanja uzoraka u SBF, kao i od vrste izabranog biomimetskog medijuma. Zaključak. Dobijanje biomimetskog kalcijum-hidroksiapatita moguće je samonukleacijom na različitim supstratima i primenom SBF.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Nucleation of biomimetic hydroxyapatite
T1  - Nukleacija biomimetskog hidroksiapatita
VL  - 58
IS  - 1
SP  - 7
EP  - 15
DO  - 10.2298/SGS1101007C
ER  - 
@article{
author = "Čolović, Božana and Marković, Dejan and Jokanović, Vukoman",
year = "2011",
abstract = "Introduction. The aim of the study was to assess the formation of biomimetic calcium hydroxyapatite (HAP) on the surface of different substrates. Material and Methods. Silica coated stainless steel tapes and thin polymer films (alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited on hydroxyapatite scaffold were used as substrate. Supersaturated simulated body fluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle's Minimum Essential Medium (EMEM) were used as bioactive liquid medium where biomimetic nucleation of HAP occurred. Infrared spectroscopy with Fourier transformation was used to analyze the formed phases, while scanning electron microscopy indicated the morphology of nucleated phase. Results. The results of measuring the mass with volume adjustments done by the BET method showed that the thickness of the film of nucleated calcium hydroxyapatite depended on the time that samples spent soaked in SBF-in as well as the type of selected biomimetic medium. Conclusion. Biomimetic calcium hydroxyapatite is possible to produce by self nucleation on different substrates in the presence of simulating body fluid., Uvod. Cilj ovog rada je bio da se ispita formiranje biomimetskog kalcijum-hidroksiapatita na površinama različitih supstrata. Materijal i metode rada. Kao supstrati su korišćeni tanki filmovi silicijum-dioksida naneti na čelične trake i tanki polimerni filmovi (alginat, celuloza, polilaktidkoglikolid) naneti na visokoporozni hidroksiapatitni nosač. Simulirana telesna tečnost (engl. simulated body fluid-SBF), kao i SBF kombinovan sa serumom iz fetusa govečeta ili Iglovim medijumom korišćeni su kao bioaktivni tečni medijum u kojem se odvija biomimetska nukleacija apatita. Za analizu formiranih faza korišćena je infracrvena spektroskopija sa Furijeovom transformacijom, dok je skening elektronska mikroskopija ukazivala na morfologiju nukleirane faze. Rezultati. Merenje mase uz korekciju zapremine primenom metode BET pokazalo je da debljina filma nukleiranog kalcijum-hidroksiapatita zavisi od vremena držanja uzoraka u SBF, kao i od vrste izabranog biomimetskog medijuma. Zaključak. Dobijanje biomimetskog kalcijum-hidroksiapatita moguće je samonukleacijom na različitim supstratima i primenom SBF.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Nucleation of biomimetic hydroxyapatite, Nukleacija biomimetskog hidroksiapatita",
volume = "58",
number = "1",
pages = "7-15",
doi = "10.2298/SGS1101007C"
}
Čolović, B., Marković, D.,& Jokanović, V.. (2011). Nucleation of biomimetic hydroxyapatite. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 58(1), 7-15.
https://doi.org/10.2298/SGS1101007C
Čolović B, Marković D, Jokanović V. Nucleation of biomimetic hydroxyapatite. in Stomatološki glasnik Srbije. 2011;58(1):7-15.
doi:10.2298/SGS1101007C .
Čolović, Božana, Marković, Dejan, Jokanović, Vukoman, "Nucleation of biomimetic hydroxyapatite" in Stomatološki glasnik Srbije, 58, no. 1 (2011):7-15,
https://doi.org/10.2298/SGS1101007C . .

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