Histopathology of subcutaneous tissue reaction to endodontic root canal sealers

Abstract

The aim of the study was to evaluate tissue response of two root canal sealers: Endomethasone (cink oxide eugenol-based sealers) and Ketac Endo Aplicap (glass ionomer based sealers). The sealers were freshly mixed and injected into subcutaneous connective tissue of the dorsal surface of experimental animals (Wistar Furth rats). The inflammatory reaction caused by the sealers was evaluated 7, 30 and 60 days after implantation using descriptive hisopathological analysis. The tissue sections were taken from injection sites. Each incision included skin,subcutaneous connective tissue and underlying muscular tissue. All blocks were proceeded using standard histological procedures. The tissue reactions were graded as mild,moderate or severe inflammation. After the seven days both sealers showed a moderate reaction with a decreasing trend. Both materials caused mild inflammation which diminished at the end of the experimental period. The results of this study demonstrate that Ketac Endo and Endomethasone is well tolerated by subcutaneus connective tissue of experimental animals.

Cilj ovih proučavanja je bio ispitivanje biokompatibilnosti dva materijala za definitivno kanalno punjenje: Endomethasone (materijal na bazi cink oksid eugenola) i Ketac Endo Aplocap (glas jonomer cementa za kanalno punjenje). Sveže pripremljeni materijali su injicirani u potkožno vezivno tkivo leđnog dela eksperimentalnih životinja (pacovi Wistar soja). Intenzitet inflamatorne reakcije proveravan je nakon 7, 30 i 60 dana na osnovu patohistološke analize. Isečak je uzet sa mesta aplikacije materijala i obuhvatao je deo kože, potkožno tkivo i deo mišićnog sloja. Tkiva su podvrgnuta standardnoj histološkoj obradi. Reakcija tkiva na ubačeni materijal označena je kao blaga, srednja i jako izražena inflamacija. Posle prvog perioda od 7 dana oba materijala su izazvala intenzivnu zapaljensku reakciju tkiva sa kasnijom tendencom smanjenja inflamacije. Oba materijala su izazvala umereno zapaljenje koje je nestalo do kraja eksperimentalnog perioda. Postignuti rezultati ukazuju da su Ketac Endo i Endomethasone biokompatibilni u potkožnom vezivnom tkivu eksperimentalnih životinja.