Antimicrobial activity of ozone gas and colloidal silver against oral microorganisms

Abstract

The aim of this study was to evaluate antimicrobial activity of ozone gas and colloidal silver suspension against selected oral microorganisms. Gaseous ozone was used in the ozone study. Suspension of reference and clinically isolated strains of oral microorganisms were exposed to ozone gas for 30, 60 and 120 s. The number of colonies was counted and the killing rate for each microorganism was calculated. In order to determine minimum inhibitory (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of colloidal silver microdilution method was used. Results showed that after 120 s exposition to gaseous ozone approximately 82.68-99.9% of all strains were eliminated. The colloidal silver exhibited strong antimicrobial activity with MICs and MBCs/MFCs values range from 2.0 to 4.0 mu g/ml. The present study confirmed the efficacy of ozone gas and colloidal silver against selected and isolated oral microorganisms. The results obtained in this study should provide additional evidence for their potential application in reducing the infection caused by microorganisms in the oral cavity.