Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant... (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativ...no visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
TY - JOUR
AU - Mitić, Aleksandar
AU - Mitić, Nadica
AU - Milašin, Jelena
AU - Živković, Slavoljub
AU - Gašić, Jovanka
AU - Mitić, Vladimir
AU - Popović, Jelena
PY - 2013
UR - https://smile.stomf.bg.ac.rs/handle/123456789/1795
AB - Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
AB - Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
PB - Srpsko lekarsko društvo, Beograd
T2 - Srpski arhiv za celokupno lekarstvo
T1 - Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
T1 - Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR
VL - 141
IS - 3-4
SP - 155
EP - 162
DO - 10.2298/SARH1304155M
ER -
@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Milašin, Jelena and Živković, Slavoljub and Gašić, Jovanka and Mitić, Vladimir and Popović, Jelena",
year = "2013",
abstract = "Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly., Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique, Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR",
volume = "141",
number = "3-4",
pages = "155-162",
doi = "10.2298/SARH1304155M"
}
Mitić, A., Mitić, N., Milašin, J., Živković, S., Gašić, J., Mitić, V.,& Popović, J.. (2013). Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 155-162.
https://doi.org/10.2298/SARH1304155M
Mitić A, Mitić N, Milašin J, Živković S, Gašić J, Mitić V, Popović J. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):155-162.
doi:10.2298/SARH1304155M .
Mitić, Aleksandar, Mitić, Nadica, Milašin, Jelena, Živković, Slavoljub, Gašić, Jovanka, Mitić, Vladimir, Popović, Jelena, "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):155-162,
https://doi.org/10.2298/SARH1304155M . .
