The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy ...promoted its toxicity.
TY - JOUR
AU - Čairović, Aleksandra
AU - Đorđević, Ivan
AU - Bulatović, Mirna
AU - Mojić, Marija
AU - Momcilović, Miljana
AU - Stošic-Grujicić, Stanislava
AU - Maksimović, Vesna
AU - Maksimović-Ivanić, Danijela
AU - Mijatović, Sanja
AU - Stamenković, Dragoslav
PY - 2013
UR - https://smile.stomf.bg.ac.rs/handle/123456789/1826
AB - The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.
T2 - Digest Journal of Nanomaterials & Biostructures
T1 - In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility
VL - 8
IS - 2
SP - 877
EP - 886
UR - https://hdl.handle.net/21.15107/rcub_vinar_5620
ER -
@article{
author = "Čairović, Aleksandra and Đorđević, Ivan and Bulatović, Mirna and Mojić, Marija and Momcilović, Miljana and Stošic-Grujicić, Stanislava and Maksimović, Vesna and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Stamenković, Dragoslav",
year = "2013",
abstract = "The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.",
journal = "Digest Journal of Nanomaterials & Biostructures",
title = "In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility",
volume = "8",
number = "2",
pages = "877-886",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5620"
}
Čairović, A., Đorđević, I., Bulatović, M., Mojić, M., Momcilović, M., Stošic-Grujicić, S., Maksimović, V., Maksimović-Ivanić, D., Mijatović, S.,& Stamenković, D.. (2013). In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility. in Digest Journal of Nanomaterials & Biostructures, 8(2), 877-886.
https://hdl.handle.net/21.15107/rcub_vinar_5620
Čairović A, Đorđević I, Bulatović M, Mojić M, Momcilović M, Stošic-Grujicić S, Maksimović V, Maksimović-Ivanić D, Mijatović S, Stamenković D. In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility. in Digest Journal of Nanomaterials & Biostructures. 2013;8(2):877-886.
https://hdl.handle.net/21.15107/rcub_vinar_5620 .
Čairović, Aleksandra, Đorđević, Ivan, Bulatović, Mirna, Mojić, Marija, Momcilović, Miljana, Stošic-Grujicić, Stanislava, Maksimović, Vesna, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Stamenković, Dragoslav, "In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility" in Digest Journal of Nanomaterials & Biostructures, 8, no. 2 (2013):877-886,
https://hdl.handle.net/21.15107/rcub_vinar_5620 .
