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Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze

dc.creatorPetrović-Matić, Sanja
dc.creatorZelić, Ksenija
dc.creatorMilašin, Jelena
dc.creatorPopović, Branka
dc.creatorPucar, Ana
dc.creatorZelić, Obrad
dc.date.accessioned2020-07-02T12:52:16Z
dc.date.available2020-07-02T12:52:16Z
dc.date.issued2014
dc.identifier.issn0370-8179
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/1917
dc.description.abstractIntroduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis.en
dc.description.abstractUvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.sr
dc.publisherSrpsko lekarsko društvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45005/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41008/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceSrpski arhiv za celokupno lekarstvo
dc.subjectperiodontitisen
dc.subjectherpes simplexen
dc.subjectgingival crevicular fluiden
dc.subjectperiodontal pocketen
dc.subjectparodontopatijasr
dc.subjectherpes simplexsr
dc.subjectgingivalna tečnostsr
dc.subjectparodontalni džepsr
dc.titleDetection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reactionen
dc.titleOtkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimerazesr
dc.typearticle
dc.rights.licenseBY-NC
dcterms.abstractЗелић, Обрад; Поповић, Бранка; Петровић-Матић, Сања; Пуцар, Aна; Зелић, Ксенија; Милашин, Јелена;
dc.citation.volume142
dc.citation.issue5-6
dc.citation.spage296
dc.citation.epage300
dc.citation.other142(5-6): 296-300
dc.citation.rankM23
dc.identifier.wos000339231200004
dc.identifier.doi10.2298/SARH1406296M
dc.identifier.scopus2-s2.0-84924241830
dc.identifier.fulltexthttps://smile.stomf.bg.ac.rs/bitstream/id/591/1912.pdf
dc.identifier.rcubconv_2331
dc.type.versionpublishedVersion


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