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Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

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Authors
Petrović, V.
Opačić-Galić, Vanja
Živković, Slavoljub
Nikolić, B.
Danilović, Vesna
Miletić, Vesna
Jokanović, Vukoman
Mitić-Ćulafić, Dragana
Article (Published version)
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Abstract
AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced ce...ll viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).

Keywords:
biocompatibility / calcium silicate / connective tissue / cytotoxicity / hydroxyapatite / inflammatory response
Source:
International Endodontic Journal, 2015, 48, 10, 966-975
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Development and Application of Methods and Materials for Monitoring New Organic Contaminants, Toxic Compounds and Heavy Metals (RS-172007)
Note:
  • Peer-reviewed manuscript: http://smile.stomf.bg.ac.rs/handle/123456789/2518

DOI: 10.1111/iej.12391

ISSN: 0143-2885

PubMed: 25288256

WoS: 000360838000008

Scopus: 2-s2.0-84940995191
[ Google Scholar ]
15
12
URI
https://smile.stomf.bg.ac.rs/handle/123456789/2012
Collections
  • Radovi istraživača
Institution/Community
Stomatološki fakultet
TY  - JOUR
AU  - Petrović, V.
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
AU  - Nikolić, B.
AU  - Danilović, Vesna
AU  - Miletić, Vesna
AU  - Jokanović, Vukoman
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2012
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 
@article{
author = "Petrović, V. and Opačić-Galić, Vanja and Živković, Slavoljub and Nikolić, B. and Danilović, Vesna and Miletić, Vesna and Jokanović, Vukoman and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}
Petrović, V., Opačić-Galić, V., Živković, S., Nikolić, B., Danilović, V., Miletić, V., Jokanović, V.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal
Wiley, Hoboken., 48(10), 966-975.
https://doi.org/10.1111/iej.12391
Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V, Jokanović V, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .
Petrović, V., Opačić-Galić, Vanja, Živković, Slavoljub, Nikolić, B., Danilović, Vesna, Miletić, Vesna, Jokanović, Vukoman, Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .

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