The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiat...ion and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.
TY - JOUR
AU - Karadžić, Ivana
AU - Vučić, Vesna
AU - Jokanović, Vukoman
AU - Debeljak-Martačić, Jasmina
AU - Marković, Dejan
AU - Petrović, Snježana
AU - Glibetić, Marija
PY - 2015
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2063
AB - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.
PB - Wiley-Blackwell, Hoboken
T2 - Journal of Biomedical Materials Research Part A
T1 - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL - 103
IS - 1
SP - 350
EP - 357
DO - 10.1002/jbm.a.35180
ER -
@article{
author = "Karadžić, Ivana and Vučić, Vesna and Jokanović, Vukoman and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}
Karadžić, I., Vučić, V., Jokanović, V., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A
Wiley-Blackwell, Hoboken., 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180
Karadžić I, Vučić V, Jokanović V, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .
Karadžić, Ivana, Vučić, Vesna, Jokanović, Vukoman, Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .
