The aim of this study was to investigate the chemical composition and antifungal activity of four essential oils from Apiaceae family, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum and Anethum graveolens, against Candida spp. reference strains and clinical isolates from human oral cavities. Chemical analysis of the essential oils (GCFID and GC/MS) showed that predominating chemical classes in the essential oil of A. graveolens was monoterpene hydrocarbons (49.4 %) and the major constituent of the oil was carvone (42.2 %): while in the oils of P. anisum, F. vulgare and P. sativa majority of compounds belong to phenylpropenes (80.9 %, 65.9 % and 49.4 %, respectively): and the major oil constituents were trans-anethole (65.1 % and 80.8 %) and myristicine (49.0 %): respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using microdilution method. All the tested oils inhibited growth of Candida spp. The strongest antifungal activity was ...shown by A. graveolens essential oil (MIC 0.63-2.5 mg mL-1; MFC 1.25-5 mg mL-1). In addition to the common use of parsnip, fennel, anise and dill in traditional medicine, present study validates use of their essential oils in treatments intended for reduction and elimination of Candida spp. population in human patients with fungal infection.
Cilj ovog istraživanja bio je da se ispita hemijski sastav i antifungalna aktivnost četiri etarska ulja familije Apiaceae, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum i Anethum graveolens, na referentne sojeve i kliničke izolate Candida spp. iz usne duplje ljudi. Hemijska analiza ispitivanih etarskih ulja (GC-FID i GC/MS) je pokazala da u uljima dominiraju sledeće hemijske klase: u ulju A. graveolens - monoterpenski ugljovodonici (49,4%) i glavni sastojak je karvon (42,2 %): dok u uljima P. anisum F. vulgare P. sativa većina jedinjenja pripada fenilpropenima (80,9 %, 65,9 % i 49,4 %, respektivno): pri čemu su glavne komponente prva dva ulja trans-anetol (65,1 % i 80.8 %): a trećeg, miristicin (49.0 %). Minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije ulja (MFC) su određene primenom mikrodilucione metode. Ispitivana tri ulja su inhibirala rast svih Candida spp.; najjaču antifungalnu aktivnost je pokazalo ulje A. graveolens (MIC 0.63-2.5 mg ml-1; MFC 1.25-5 mg... ml-1). Pored česte primene paškanata, morača, anisa i mirođije u tradicionalnoj medicini, ova studija potvrđuje upotrebu njihovih etarskih ulja u tretmanima redukcije i eliminisanju populacije Candida spp. kod humanih pacijenata sa gljivičnim infekcijama.
TY - JOUR
AU - Nikolić, Miloš
AU - Marković, Tatjana
AU - Ćirić, Ana
AU - Glamočlija, Jasmina
AU - Marković, Dejan
AU - Soković, Marina
PY - 2015
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2065
AB - The aim of this study was to investigate the chemical composition and antifungal activity of four essential oils from Apiaceae family, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum and Anethum graveolens, against Candida spp. reference strains and clinical isolates from human oral cavities. Chemical analysis of the essential oils (GCFID and GC/MS) showed that predominating chemical classes in the essential oil of A. graveolens was monoterpene hydrocarbons (49.4 %) and the major constituent of the oil was carvone (42.2 %): while in the oils of P. anisum, F. vulgare and P. sativa majority of compounds belong to phenylpropenes (80.9 %, 65.9 % and 49.4 %, respectively): and the major oil constituents were trans-anethole (65.1 % and 80.8 %) and myristicine (49.0 %): respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using microdilution method. All the tested oils inhibited growth of Candida spp. The strongest antifungal activity was shown by A. graveolens essential oil (MIC 0.63-2.5 mg mL-1; MFC 1.25-5 mg mL-1). In addition to the common use of parsnip, fennel, anise and dill in traditional medicine, present study validates use of their essential oils in treatments intended for reduction and elimination of Candida spp. population in human patients with fungal infection.
AB - Cilj ovog istraživanja bio je da se ispita hemijski sastav i antifungalna aktivnost četiri etarska ulja familije Apiaceae, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum i Anethum graveolens, na referentne sojeve i kliničke izolate Candida spp. iz usne duplje ljudi. Hemijska analiza ispitivanih etarskih ulja (GC-FID i GC/MS) je pokazala da u uljima dominiraju sledeće hemijske klase: u ulju A. graveolens - monoterpenski ugljovodonici (49,4%) i glavni sastojak je karvon (42,2 %): dok u uljima P. anisum F. vulgare P. sativa većina jedinjenja pripada fenilpropenima (80,9 %, 65,9 % i 49,4 %, respektivno): pri čemu su glavne komponente prva dva ulja trans-anetol (65,1 % i 80.8 %): a trećeg, miristicin (49.0 %). Minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije ulja (MFC) su određene primenom mikrodilucione metode. Ispitivana tri ulja su inhibirala rast svih Candida spp.; najjaču antifungalnu aktivnost je pokazalo ulje A. graveolens (MIC 0.63-2.5 mg ml-1; MFC 1.25-5 mg ml-1). Pored česte primene paškanata, morača, anisa i mirođije u tradicionalnoj medicini, ova studija potvrđuje upotrebu njihovih etarskih ulja u tretmanima redukcije i eliminisanju populacije Candida spp. kod humanih pacijenata sa gljivičnim infekcijama.
PB - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2 - Lekovite sirovine
T1 - Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species
T1 - Osetljivost referentnih sojeva i oralnih kliničkih izolata Candida spp. - na odabrana etarska ulja familije Apiaceae
IS - 35
SP - 151
EP - 162
DO - 10.5937/leksir1535151N
ER -
@article{
author = "Nikolić, Miloš and Marković, Tatjana and Ćirić, Ana and Glamočlija, Jasmina and Marković, Dejan and Soković, Marina",
year = "2015",
abstract = "The aim of this study was to investigate the chemical composition and antifungal activity of four essential oils from Apiaceae family, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum and Anethum graveolens, against Candida spp. reference strains and clinical isolates from human oral cavities. Chemical analysis of the essential oils (GCFID and GC/MS) showed that predominating chemical classes in the essential oil of A. graveolens was monoterpene hydrocarbons (49.4 %) and the major constituent of the oil was carvone (42.2 %): while in the oils of P. anisum, F. vulgare and P. sativa majority of compounds belong to phenylpropenes (80.9 %, 65.9 % and 49.4 %, respectively): and the major oil constituents were trans-anethole (65.1 % and 80.8 %) and myristicine (49.0 %): respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using microdilution method. All the tested oils inhibited growth of Candida spp. The strongest antifungal activity was shown by A. graveolens essential oil (MIC 0.63-2.5 mg mL-1; MFC 1.25-5 mg mL-1). In addition to the common use of parsnip, fennel, anise and dill in traditional medicine, present study validates use of their essential oils in treatments intended for reduction and elimination of Candida spp. population in human patients with fungal infection., Cilj ovog istraživanja bio je da se ispita hemijski sastav i antifungalna aktivnost četiri etarska ulja familije Apiaceae, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum i Anethum graveolens, na referentne sojeve i kliničke izolate Candida spp. iz usne duplje ljudi. Hemijska analiza ispitivanih etarskih ulja (GC-FID i GC/MS) je pokazala da u uljima dominiraju sledeće hemijske klase: u ulju A. graveolens - monoterpenski ugljovodonici (49,4%) i glavni sastojak je karvon (42,2 %): dok u uljima P. anisum F. vulgare P. sativa većina jedinjenja pripada fenilpropenima (80,9 %, 65,9 % i 49,4 %, respektivno): pri čemu su glavne komponente prva dva ulja trans-anetol (65,1 % i 80.8 %): a trećeg, miristicin (49.0 %). Minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije ulja (MFC) su određene primenom mikrodilucione metode. Ispitivana tri ulja su inhibirala rast svih Candida spp.; najjaču antifungalnu aktivnost je pokazalo ulje A. graveolens (MIC 0.63-2.5 mg ml-1; MFC 1.25-5 mg ml-1). Pored česte primene paškanata, morača, anisa i mirođije u tradicionalnoj medicini, ova studija potvrđuje upotrebu njihovih etarskih ulja u tretmanima redukcije i eliminisanju populacije Candida spp. kod humanih pacijenata sa gljivičnim infekcijama.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species, Osetljivost referentnih sojeva i oralnih kliničkih izolata Candida spp. - na odabrana etarska ulja familije Apiaceae",
number = "35",
pages = "151-162",
doi = "10.5937/leksir1535151N"
}
Nikolić, M., Marković, T., Ćirić, A., Glamočlija, J., Marković, D.,& Soković, M.. (2015). Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(35), 151-162.
https://doi.org/10.5937/leksir1535151N
Nikolić M, Marković T, Ćirić A, Glamočlija J, Marković D, Soković M. Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species. in Lekovite sirovine. 2015;(35):151-162.
doi:10.5937/leksir1535151N .
Nikolić, Miloš, Marković, Tatjana, Ćirić, Ana, Glamočlija, Jasmina, Marković, Dejan, Soković, Marina, "Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species" in Lekovite sirovine, no. 35 (2015):151-162,
https://doi.org/10.5937/leksir1535151N . .
