SMILE – Repository of the Faculty of Dental Medicine
University of Belgrade - Faculty of Dental Medicine
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   SMILE
  • Stomatološki fakultet
  • Radovi istraživača
  • View Item
  •   SMILE
  • Stomatološki fakultet
  • Radovi istraživača
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma

Thumbnail
2016
2110.pdf (1.600Mb)
Authors
Čarkić, Jelena
Nikolić, Nadja
Radojević-Škodrić, Sanja
Kuzmanović-Pfićer, Jovana
Brajović, Gavrilo
Antunović, Marija
Milašin, Jelena
Popović, Branka
Article (Published version)
Metadata
Show full item record
Abstract
The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend t...owards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.

Keywords:
OSCC / TERT-CLPTM1L / telomere length / telomerase
Source:
Journal of Oral Science, 2016, 58, 4, 449-458
Publisher:
  • Nihon Univ, School Dentistry, Toyko
Funding / projects:
  • Genetic control and molecular mechanisms in malignant, inflammatory and developmental pathologies of the orofacial region (RS-175075)

DOI: 10.2334/josnusd.16-0108

ISSN: 1343-4934

PubMed: 28025427

WoS: 000390087600001

Scopus: 2-s2.0-85007377411
[ Google Scholar ]
12
11
URI
https://smile.stomf.bg.ac.rs/handle/123456789/2115
Collections
  • Radovi istraživača
Institution/Community
Stomatološki fakultet
TY  - JOUR
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Radojević-Škodrić, Sanja
AU  - Kuzmanović-Pfićer, Jovana
AU  - Brajović, Gavrilo
AU  - Antunović, Marija
AU  - Milašin, Jelena
AU  - Popović, Branka
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2115
AB  - The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.
PB  - Nihon Univ, School Dentistry, Toyko
T2  - Journal of Oral Science
T1  - The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma
VL  - 58
IS  - 4
SP  - 449
EP  - 458
DO  - 10.2334/josnusd.16-0108
ER  - 
@article{
author = "Čarkić, Jelena and Nikolić, Nadja and Radojević-Škodrić, Sanja and Kuzmanović-Pfićer, Jovana and Brajović, Gavrilo and Antunović, Marija and Milašin, Jelena and Popović, Branka",
year = "2016",
abstract = "The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.",
publisher = "Nihon Univ, School Dentistry, Toyko",
journal = "Journal of Oral Science",
title = "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma",
volume = "58",
number = "4",
pages = "449-458",
doi = "10.2334/josnusd.16-0108"
}
Čarkić, J., Nikolić, N., Radojević-Škodrić, S., Kuzmanović-Pfićer, J., Brajović, G., Antunović, M., Milašin, J.,& Popović, B.. (2016). The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science
Nihon Univ, School Dentistry, Toyko., 58(4), 449-458.
https://doi.org/10.2334/josnusd.16-0108
Čarkić J, Nikolić N, Radojević-Škodrić S, Kuzmanović-Pfićer J, Brajović G, Antunović M, Milašin J, Popović B. The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science. 2016;58(4):449-458.
doi:10.2334/josnusd.16-0108 .
Čarkić, Jelena, Nikolić, Nadja, Radojević-Škodrić, Sanja, Kuzmanović-Pfićer, Jovana, Brajović, Gavrilo, Antunović, Marija, Milašin, Jelena, Popović, Branka, "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma" in Journal of Oral Science, 58, no. 4 (2016):449-458,
https://doi.org/10.2334/josnusd.16-0108 . .

DSpace software copyright © 2002-2015  DuraSpace
About Smile – School of dental Medicine dIgitaL archivE | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About Smile – School of dental Medicine dIgitaL archivE | Send Feedback

OpenAIRERCUB