The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma

2016
Authors
Čarkić, JelenaNikolić, Nadja

Radojević-Škodrić, Sanja
Kuzmanović-Pfićer, Jovana
Brajović, Gavrilo

Antunović, Marija
Milašin, Jelena

Popović, Branka

Article (Published version)

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The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend t...owards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.
Keywords:
OSCC / TERT-CLPTM1L / telomere length / telomeraseSource:
Journal of Oral Science, 2016, 58, 4, 449-458Publisher:
- Nihon Univ, School Dentistry, Toyko
Funding / projects:
DOI: 10.2334/josnusd.16-0108
ISSN: 1343-4934
PubMed: 28025427
WoS: 000390087600001
Scopus: 2-s2.0-85007377411
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Stomatološki fakultetTY - JOUR AU - Čarkić, Jelena AU - Nikolić, Nadja AU - Radojević-Škodrić, Sanja AU - Kuzmanović-Pfićer, Jovana AU - Brajović, Gavrilo AU - Antunović, Marija AU - Milašin, Jelena AU - Popović, Branka PY - 2016 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2115 AB - The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers. PB - Nihon Univ, School Dentistry, Toyko T2 - Journal of Oral Science T1 - The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma VL - 58 IS - 4 SP - 449 EP - 458 DO - 10.2334/josnusd.16-0108 ER -
@article{ author = "Čarkić, Jelena and Nikolić, Nadja and Radojević-Škodrić, Sanja and Kuzmanović-Pfićer, Jovana and Brajović, Gavrilo and Antunović, Marija and Milašin, Jelena and Popović, Branka", year = "2016", abstract = "The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.", publisher = "Nihon Univ, School Dentistry, Toyko", journal = "Journal of Oral Science", title = "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma", volume = "58", number = "4", pages = "449-458", doi = "10.2334/josnusd.16-0108" }
Čarkić, J., Nikolić, N., Radojević-Škodrić, S., Kuzmanović-Pfićer, J., Brajović, G., Antunović, M., Milašin, J.,& Popović, B.. (2016). The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science Nihon Univ, School Dentistry, Toyko., 58(4), 449-458. https://doi.org/10.2334/josnusd.16-0108
Čarkić J, Nikolić N, Radojević-Škodrić S, Kuzmanović-Pfićer J, Brajović G, Antunović M, Milašin J, Popović B. The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science. 2016;58(4):449-458. doi:10.2334/josnusd.16-0108 .
Čarkić, Jelena, Nikolić, Nadja, Radojević-Škodrić, Sanja, Kuzmanović-Pfićer, Jovana, Brajović, Gavrilo, Antunović, Marija, Milašin, Jelena, Popović, Branka, "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma" in Journal of Oral Science, 58, no. 4 (2016):449-458, https://doi.org/10.2334/josnusd.16-0108 . .