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Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study

Authorized Users Only
2016
Authors
Delgado-Ruiz, Rafael
Gomez-Moreno, Gerardo
Aguilar-Salvatierra, Antonio
Marković, Aleksa
Eduardo Mate-Sanchez, Jose
Calvo Guirado, Jose Luis
Article (Published version)
Metadata
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Abstract
ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were eva...luated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.

Keywords:
Alizarin / alkaline phosphatase / cell density / dental implants / femtosecond laser / human fetal osteoblast / microgrooved surfaces / Zirconia
Source:
Clinical Oral Implants Research, 2016, 27, 11, e144-e153
Publisher:
  • Wiley, Hoboken

DOI: 10.1111/clr.12585

ISSN: 0905-7161

PubMed: 25809053

WoS: 000387071900024

Scopus: 2-s2.0-84926513083
[ Google Scholar ]
31
21
URI
https://smile.stomf.bg.ac.rs/handle/123456789/2125
Collections
  • Radovi istraživača
Institution/Community
Stomatološki fakultet
TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Gomez-Moreno, Gerardo
AU  - Aguilar-Salvatierra, Antonio
AU  - Marković, Aleksa
AU  - Eduardo Mate-Sanchez, Jose
AU  - Calvo Guirado, Jose Luis
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2125
AB  - ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.
PB  - Wiley, Hoboken
T2  - Clinical Oral Implants Research
T1  - Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study
VL  - 27
IS  - 11
SP  - e144
EP  - e153
DO  - 10.1111/clr.12585
ER  - 
@article{
author = "Delgado-Ruiz, Rafael and Gomez-Moreno, Gerardo and Aguilar-Salvatierra, Antonio and Marković, Aleksa and Eduardo Mate-Sanchez, Jose and Calvo Guirado, Jose Luis",
year = "2016",
abstract = "ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.",
publisher = "Wiley, Hoboken",
journal = "Clinical Oral Implants Research",
title = "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study",
volume = "27",
number = "11",
pages = "e144-e153",
doi = "10.1111/clr.12585"
}
Delgado-Ruiz, R., Gomez-Moreno, G., Aguilar-Salvatierra, A., Marković, A., Eduardo Mate-Sanchez, J.,& Calvo Guirado, J. L.. (2016). Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research
Wiley, Hoboken., 27(11), e144-e153.
https://doi.org/10.1111/clr.12585
Delgado-Ruiz R, Gomez-Moreno G, Aguilar-Salvatierra A, Marković A, Eduardo Mate-Sanchez J, Calvo Guirado JL. Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research. 2016;27(11):e144-e153.
doi:10.1111/clr.12585 .
Delgado-Ruiz, Rafael, Gomez-Moreno, Gerardo, Aguilar-Salvatierra, Antonio, Marković, Aleksa, Eduardo Mate-Sanchez, Jose, Calvo Guirado, Jose Luis, "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study" in Clinical Oral Implants Research, 27, no. 11 (2016):e144-e153,
https://doi.org/10.1111/clr.12585 . .

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