The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group.... In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
Keywords:
Acute inflammation / angular second moment / correlation / fractal dimension
TY - JOUR
AU - Stanković, Marija
AU - Pantić, Igor
AU - de Luka, Silvio R.
AU - Puškaš, Nela
AU - Zaletel, Ivan
AU - Milutinović-Smiljanić, Sanja
AU - Pantić, Senka
AU - Trbovich, Alexander M.
PY - 2016
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2129
AB - The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
PB - Wiley, Hoboken
T2 - Journal of Microscopy
T1 - Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation
VL - 261
IS - 3
SP - 277
EP - 284
DO - 10.1111/jmi.12330
ER -
@article{
author = "Stanković, Marija and Pantić, Igor and de Luka, Silvio R. and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Pantić, Senka and Trbovich, Alexander M.",
year = "2016",
abstract = "The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Microscopy",
title = "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation",
volume = "261",
number = "3",
pages = "277-284",
doi = "10.1111/jmi.12330"
}
Stanković, M., Pantić, I., de Luka, S. R., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S., Pantić, S.,& Trbovich, A. M.. (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy
Wiley, Hoboken., 261(3), 277-284.
https://doi.org/10.1111/jmi.12330
Stanković M, Pantić I, de Luka SR, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Pantić S, Trbovich AM. Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy. 2016;261(3):277-284.
doi:10.1111/jmi.12330 .
Stanković, Marija, Pantić, Igor, de Luka, Silvio R., Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Pantić, Senka, Trbovich, Alexander M., "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation" in Journal of Microscopy, 261, no. 3 (2016):277-284,
https://doi.org/10.1111/jmi.12330 . .
