The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma
Само за регистроване кориснике
2016
Аутори
Jelovac, DragoTepavčević, Zvezdana
Nikolić, Nadja
Ilić, Branislav
Eljabo, Najib
Popović, Branka
Čarkić, Jelena
Konstantinović, Vitomir
Vukadinović, Miroslav
Miličić, Biljana
Milašin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The tumour subtype, TNM classification, and histopathological data are sometimes not sufficient for understanding and assessing the behaviour of oral cancers. In an attempt to find additional markers of tumour biology and behaviour, this study sought to determine the incidence and consequently the relevance of c-erb-B2, c-Myc, and H-ras gene alterations in tumour-free margins of oral squamous cell carcinoma (OSCC). Fifty samples of OSCC were analyzed for c-erb-B2 and c-Myc amplification by real-time polymerase chain reaction and for H-ras point mutations by sequencing. A relatively high incidence of genetic lesions was detected: 22% of cases had c-erb-B2 and 30% had c-Myc amplification, whilst only 12% harboured H-ras mutations. Kaplan-Meier analysis and the log-rank test showed statistically significant differences in 5-year survival rates and relapse between patients with tumour margins positive for c-erb-B2 amplification and those with margins that were negative (P = 0.002). H-ras a...nd c-Myc alterations could not be associated with tumour behaviour. Molecular analysis of margins, targeting cancer genes, could identify additional, independent predictors of risk and outcome in OSCC.
Кључне речи:
oral cancer / oncogenes / H-ras / c-Myc / c-erb-B2 / mutation / amplificationИзвор:
International Journal of Oral & Maxillofacial Surgery, 2016, 45, 6, 700-705Издавач:
- Churchill Livingstone, Edinburgh
Финансирање / пројекти:
- Генетичка контрола и молекуларни механизми у малигним, инфламаторним и развојним патологијама орофацијалне регије (RS-MESTD-Basic Research (BR or ON)-175075)
DOI: 10.1016/j.ijom.2015.11.014
ISSN: 0901-5027
PubMed: 26708050
WoS: 000377232800005
Scopus: 2-s2.0-84950124398
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Jelovac, Drago AU - Tepavčević, Zvezdana AU - Nikolić, Nadja AU - Ilić, Branislav AU - Eljabo, Najib AU - Popović, Branka AU - Čarkić, Jelena AU - Konstantinović, Vitomir AU - Vukadinović, Miroslav AU - Miličić, Biljana AU - Milašin, Jelena PY - 2016 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2180 AB - The tumour subtype, TNM classification, and histopathological data are sometimes not sufficient for understanding and assessing the behaviour of oral cancers. In an attempt to find additional markers of tumour biology and behaviour, this study sought to determine the incidence and consequently the relevance of c-erb-B2, c-Myc, and H-ras gene alterations in tumour-free margins of oral squamous cell carcinoma (OSCC). Fifty samples of OSCC were analyzed for c-erb-B2 and c-Myc amplification by real-time polymerase chain reaction and for H-ras point mutations by sequencing. A relatively high incidence of genetic lesions was detected: 22% of cases had c-erb-B2 and 30% had c-Myc amplification, whilst only 12% harboured H-ras mutations. Kaplan-Meier analysis and the log-rank test showed statistically significant differences in 5-year survival rates and relapse between patients with tumour margins positive for c-erb-B2 amplification and those with margins that were negative (P = 0.002). H-ras and c-Myc alterations could not be associated with tumour behaviour. Molecular analysis of margins, targeting cancer genes, could identify additional, independent predictors of risk and outcome in OSCC. PB - Churchill Livingstone, Edinburgh T2 - International Journal of Oral & Maxillofacial Surgery T1 - The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma VL - 45 IS - 6 SP - 700 EP - 705 DO - 10.1016/j.ijom.2015.11.014 ER -
@article{ author = "Jelovac, Drago and Tepavčević, Zvezdana and Nikolić, Nadja and Ilić, Branislav and Eljabo, Najib and Popović, Branka and Čarkić, Jelena and Konstantinović, Vitomir and Vukadinović, Miroslav and Miličić, Biljana and Milašin, Jelena", year = "2016", abstract = "The tumour subtype, TNM classification, and histopathological data are sometimes not sufficient for understanding and assessing the behaviour of oral cancers. In an attempt to find additional markers of tumour biology and behaviour, this study sought to determine the incidence and consequently the relevance of c-erb-B2, c-Myc, and H-ras gene alterations in tumour-free margins of oral squamous cell carcinoma (OSCC). Fifty samples of OSCC were analyzed for c-erb-B2 and c-Myc amplification by real-time polymerase chain reaction and for H-ras point mutations by sequencing. A relatively high incidence of genetic lesions was detected: 22% of cases had c-erb-B2 and 30% had c-Myc amplification, whilst only 12% harboured H-ras mutations. Kaplan-Meier analysis and the log-rank test showed statistically significant differences in 5-year survival rates and relapse between patients with tumour margins positive for c-erb-B2 amplification and those with margins that were negative (P = 0.002). H-ras and c-Myc alterations could not be associated with tumour behaviour. Molecular analysis of margins, targeting cancer genes, could identify additional, independent predictors of risk and outcome in OSCC.", publisher = "Churchill Livingstone, Edinburgh", journal = "International Journal of Oral & Maxillofacial Surgery", title = "The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma", volume = "45", number = "6", pages = "700-705", doi = "10.1016/j.ijom.2015.11.014" }
Jelovac, D., Tepavčević, Z., Nikolić, N., Ilić, B., Eljabo, N., Popović, B., Čarkić, J., Konstantinović, V., Vukadinović, M., Miličić, B.,& Milašin, J.. (2016). The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery Churchill Livingstone, Edinburgh., 45(6), 700-705. https://doi.org/10.1016/j.ijom.2015.11.014
Jelovac D, Tepavčević Z, Nikolić N, Ilić B, Eljabo N, Popović B, Čarkić J, Konstantinović V, Vukadinović M, Miličić B, Milašin J. The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery. 2016;45(6):700-705. doi:10.1016/j.ijom.2015.11.014 .
Jelovac, Drago, Tepavčević, Zvezdana, Nikolić, Nadja, Ilić, Branislav, Eljabo, Najib, Popović, Branka, Čarkić, Jelena, Konstantinović, Vitomir, Vukadinović, Miroslav, Miličić, Biljana, Milašin, Jelena, "The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma" in International Journal of Oral & Maxillofacial Surgery, 45, no. 6 (2016):700-705, https://doi.org/10.1016/j.ijom.2015.11.014 . .