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dc.creatorRadunović, Milena
dc.creatorDe Colli, Marianna
dc.creatorDe Marco, Patrizia
dc.creatorDi Nisio, Chiara
dc.creatorFontana, Antonella
dc.creatorPiattelli, Adriano
dc.creatorCataldi, Amelia
dc.creatorZara, Susi
dc.date.accessioned2020-07-02T13:09:12Z
dc.date.available2020-07-02T13:09:12Z
dc.date.issued2017
dc.identifier.issn1549-3296
dc.identifier.urihttp://smile.stomf.bg.ac.rs/handle/123456789/2186
dc.description.abstractCollagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.en
dc.publisherWiley, Hoboken
dc.relationMIUR FIRB-Accordi di Programma ProjectMinistry of Education, Universities and Research (MIUR)
dc.relationPO FSE Abruzzo -Progetto "Innovazione, Sviluppo e Competitivita"
dc.relationReti per l'alta formazione
dc.rightsrestrictedAccess
dc.sourceJournal of Biomedical Materials Research Part A
dc.subjectgraphene oxideen
dc.subjectcollagenous membranesen
dc.subjectDPSCsen
dc.subjectdifferentiationen
dc.subjectbiocompatibilityen
dc.titleGraphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrenceen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЦаталди, Aмелиа; Пиаттелли, Aдриано; Де Марцо, Патризиа; Фонтана, Aнтонелла; Зара, Суси; Радуновић, Милена; Де Цолли, Марианна; Ди Нисио, Цхиара;
dc.citation.volume105
dc.citation.issue8
dc.citation.spage2312
dc.citation.epage2320
dc.citation.other105(8): 2312-2320
dc.citation.rankM21
dc.identifier.wos000404370600020
dc.identifier.doi10.1002/jbm.a.36085
dc.identifier.pmid28380697
dc.identifier.scopus2-s2.0-85019736830
dc.identifier.rcubconv_3456
dc.type.versionpublishedVersion


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