Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma
Samo za registrovane korisnike
2018
Autori
Lazarević, MilošMilošević, Maja
Trišić, Dijana
Toljić, Boško
Simonović, Jelena
Nikolić, Nadja
Miković, Nikola
Jelovac, Drago
Petrović, Milan
Vukadinović, Miroslav
Milašin, Jelena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originati...ng from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.
Ključne reči:
cancer stem cells / embryonic and mesenchymal markers / oral squamous cell carcinoma / surgical marginIzvor:
Journal of BUON, 2018, 23, 6, 1686-1692Izdavač:
- Balkan Union of Oncology (B.U.ON.)
Finansiranje / projekti:
- Genetička kontrola i molekularni mehanizmi u malignim, inflamatornim i razvojnim patologijama orofacijalne regije (RS-MESTD-Basic Research (BR or ON)-175075)
- Ministry of Foreign Affairs of Italy [PGR02954]
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Lazarević, Miloš AU - Milošević, Maja AU - Trišić, Dijana AU - Toljić, Boško AU - Simonović, Jelena AU - Nikolić, Nadja AU - Miković, Nikola AU - Jelovac, Drago AU - Petrović, Milan AU - Vukadinović, Miroslav AU - Milašin, Jelena PY - 2018 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2271 AB - Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of BUON T1 - Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma VL - 23 IS - 6 SP - 1686 EP - 1692 UR - https://hdl.handle.net/21.15107/rcub_smile_2271 ER -
@article{ author = "Lazarević, Miloš and Milošević, Maja and Trišić, Dijana and Toljić, Boško and Simonović, Jelena and Nikolić, Nadja and Miković, Nikola and Jelovac, Drago and Petrović, Milan and Vukadinović, Miroslav and Milašin, Jelena", year = "2018", abstract = "Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of BUON", title = "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma", volume = "23", number = "6", pages = "1686-1692", url = "https://hdl.handle.net/21.15107/rcub_smile_2271" }
Lazarević, M., Milošević, M., Trišić, D., Toljić, B., Simonović, J., Nikolić, N., Miković, N., Jelovac, D., Petrović, M., Vukadinović, M.,& Milašin, J.. (2018). Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON Balkan Union of Oncology (B.U.ON.)., 23(6), 1686-1692. https://hdl.handle.net/21.15107/rcub_smile_2271
Lazarević M, Milošević M, Trišić D, Toljić B, Simonović J, Nikolić N, Miković N, Jelovac D, Petrović M, Vukadinović M, Milašin J. Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON. 2018;23(6):1686-1692. https://hdl.handle.net/21.15107/rcub_smile_2271 .
Lazarević, Miloš, Milošević, Maja, Trišić, Dijana, Toljić, Boško, Simonović, Jelena, Nikolić, Nadja, Miković, Nikola, Jelovac, Drago, Petrović, Milan, Vukadinović, Miroslav, Milašin, Jelena, "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma" in Journal of BUON, 23, no. 6 (2018):1686-1692, https://hdl.handle.net/21.15107/rcub_smile_2271 .