BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed hig...her chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.
TY - JOUR
AU - Milošević, Maja
AU - Lazarević, Miloš
AU - Toljić, Boško
AU - Simonović, Jelena
AU - Trišić, Dijana
AU - Nikolić, Nadja
AU - Petrović, Milan
AU - Milašin, Jelena
PY - 2018
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2279
AB - BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.
PB - Wiley, Hoboken
T2 - Experimental Dermatology
T1 - Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins
VL - 27
IS - 10
SP - 1160
EP - 1165
DO - 10.1111/exd.13755
ER -
@article{
author = "Milošević, Maja and Lazarević, Miloš and Toljić, Boško and Simonović, Jelena and Trišić, Dijana and Nikolić, Nadja and Petrović, Milan and Milašin, Jelena",
year = "2018",
abstract = "BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.",
publisher = "Wiley, Hoboken",
journal = "Experimental Dermatology",
title = "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins",
volume = "27",
number = "10",
pages = "1160-1165",
doi = "10.1111/exd.13755"
}
Milošević, M., Lazarević, M., Toljić, B., Simonović, J., Trišić, D., Nikolić, N., Petrović, M.,& Milašin, J.. (2018). Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology
Wiley, Hoboken., 27(10), 1160-1165.
https://doi.org/10.1111/exd.13755
Milošević M, Lazarević M, Toljić B, Simonović J, Trišić D, Nikolić N, Petrović M, Milašin J. Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology. 2018;27(10):1160-1165.
doi:10.1111/exd.13755 .
Milošević, Maja, Lazarević, Miloš, Toljić, Boško, Simonović, Jelena, Trišić, Dijana, Nikolić, Nadja, Petrović, Milan, Milašin, Jelena, "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins" in Experimental Dermatology, 27, no. 10 (2018):1160-1165,
https://doi.org/10.1111/exd.13755 . .
