Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis
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2018
Authors
Jakovljević, Aleksandar
Knežević, Aleksandra

Nikolić, Nadja

Soldatović, Ivan

Jovanović, T.
Milašin, Jelena

Andrić, Miroslav

Article (Published version)

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ObjectivesThis study aimed to analyse Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-, IL-1 and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines. Materials and MethodsA total of 100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-, IL-1 and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis. ResultsThere were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (p=.686, p=.879, respectively). Only 12 of 74 EBV (16.2%) and four of 54 HCMV (13.5%) nested PCR-positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significa...nt correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample. ConclusionThe observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines.
Keywords:
apical periodontitis / Epstein-Barr virus / human cytomegalovirus / interleukin-1beta / interleukin-6 / tumour necrosis factor-alphaSource:
Oral Diseases, 2018, 24, 5, 840-846Publisher:
- Wiley, Hoboken
Funding / projects:
DOI: 10.1111/odi.12829
ISSN: 1354-523X
PubMed: 29316040
WoS: 000435803800020
Scopus: 2-s2.0-85043509314
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Stomatološki fakultetTY - JOUR AU - Jakovljević, Aleksandar AU - Knežević, Aleksandra AU - Nikolić, Nadja AU - Soldatović, Ivan AU - Jovanović, T. AU - Milašin, Jelena AU - Andrić, Miroslav PY - 2018 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2296 AB - ObjectivesThis study aimed to analyse Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-, IL-1 and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines. Materials and MethodsA total of 100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-, IL-1 and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis. ResultsThere were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (p=.686, p=.879, respectively). Only 12 of 74 EBV (16.2%) and four of 54 HCMV (13.5%) nested PCR-positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample. ConclusionThe observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines. PB - Wiley, Hoboken T2 - Oral Diseases T1 - Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis VL - 24 IS - 5 SP - 840 EP - 846 DO - 10.1111/odi.12829 ER -
@article{ author = "Jakovljević, Aleksandar and Knežević, Aleksandra and Nikolić, Nadja and Soldatović, Ivan and Jovanović, T. and Milašin, Jelena and Andrić, Miroslav", year = "2018", abstract = "ObjectivesThis study aimed to analyse Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-, IL-1 and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines. Materials and MethodsA total of 100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-, IL-1 and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis. ResultsThere were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (p=.686, p=.879, respectively). Only 12 of 74 EBV (16.2%) and four of 54 HCMV (13.5%) nested PCR-positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample. ConclusionThe observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines.", publisher = "Wiley, Hoboken", journal = "Oral Diseases", title = "Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis", volume = "24", number = "5", pages = "840-846", doi = "10.1111/odi.12829" }
Jakovljević, A., Knežević, A., Nikolić, N., Soldatović, I., Jovanović, T., Milašin, J.,& Andrić, M.. (2018). Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis. in Oral Diseases Wiley, Hoboken., 24(5), 840-846. https://doi.org/10.1111/odi.12829
Jakovljević A, Knežević A, Nikolić N, Soldatović I, Jovanović T, Milašin J, Andrić M. Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis. in Oral Diseases. 2018;24(5):840-846. doi:10.1111/odi.12829 .
Jakovljević, Aleksandar, Knežević, Aleksandra, Nikolić, Nadja, Soldatović, Ivan, Jovanović, T., Milašin, Jelena, Andrić, Miroslav, "Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis" in Oral Diseases, 24, no. 5 (2018):840-846, https://doi.org/10.1111/odi.12829 . .