Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, su...ch as implant stability loss and further surgical procedures, are reduced.
MIUR FIRB-Accordi di Programma Project Processi degenerativi dei tessuti mineralizzati del cavo orale, impiego di biomateriali e controllo delle interazioni con i microrganismi dell'ambiente', CARICHIETI
PO FSE Abruzzo - Progetto "Innovazione, Sviluppo e Competitivita"
TY - JOUR
AU - De Colli, Marianna
AU - Radunović, Milena
AU - Zizzari, Vincenzo L.
AU - Di Giacomo, Viviana
AU - Di Nisio, Chiara
AU - Piattelli, Adriano
AU - Calvo Guirado, Jose Luis
AU - Zavan, Barbara
AU - Cataldi, Amelia
AU - Zara, Susi
PY - 2018
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2304
AB - Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.
PB - Japanese Soc Dental Materials Devices, Tokyo
T2 - Dental Materials Journal
T1 - Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface
VL - 37
IS - 2
SP - 197
EP - 205
DO - 10.4012/dmj.2016-418
ER -
@article{
author = "De Colli, Marianna and Radunović, Milena and Zizzari, Vincenzo L. and Di Giacomo, Viviana and Di Nisio, Chiara and Piattelli, Adriano and Calvo Guirado, Jose Luis and Zavan, Barbara and Cataldi, Amelia and Zara, Susi",
year = "2018",
abstract = "Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.",
publisher = "Japanese Soc Dental Materials Devices, Tokyo",
journal = "Dental Materials Journal",
title = "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface",
volume = "37",
number = "2",
pages = "197-205",
doi = "10.4012/dmj.2016-418"
}
De Colli, M., Radunović, M., Zizzari, V. L., Di Giacomo, V., Di Nisio, C., Piattelli, A., Calvo Guirado, J. L., Zavan, B., Cataldi, A.,& Zara, S.. (2018). Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal
Japanese Soc Dental Materials Devices, Tokyo., 37(2), 197-205.
https://doi.org/10.4012/dmj.2016-418
De Colli M, Radunović M, Zizzari VL, Di Giacomo V, Di Nisio C, Piattelli A, Calvo Guirado JL, Zavan B, Cataldi A, Zara S. Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal. 2018;37(2):197-205.
doi:10.4012/dmj.2016-418 .
De Colli, Marianna, Radunović, Milena, Zizzari, Vincenzo L., Di Giacomo, Viviana, Di Nisio, Chiara, Piattelli, Adriano, Calvo Guirado, Jose Luis, Zavan, Barbara, Cataldi, Amelia, Zara, Susi, "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface" in Dental Materials Journal, 37, no. 2 (2018):197-205,
https://doi.org/10.4012/dmj.2016-418 . .
