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dc.creatorSimonović, Jelena
dc.creatorToljić, Boško
dc.creatorNikolić, Nadja
dc.creatorPerić, Mina
dc.creatorVujin, Jasna
dc.creatorPanajotović, Radmila
dc.creatorGajić, Radoš
dc.creatorBekyarova, Elena
dc.creatorCataldi, Amelia
dc.creatorParpura, Vladimir
dc.creatorMilašin, Jelena
dc.date.accessioned2020-07-02T13:18:09Z
dc.date.available2020-07-02T13:18:09Z
dc.date.issued2018
dc.identifier.issn1549-3296
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/2327
dc.description.abstractStem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175075/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Biomedical Materials Research Part A
dc.subjectstem cellsen
dc.subjectapical papillaen
dc.subjectneural inductionen
dc.subjectgraphene dispersionen
dc.subjectcarbon nanotubesen
dc.titleDifferentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПанајотовић, Радмила; Вујин, Јасна; Перић, Мина; Цаталди, Aмелиа; Симоновић, Јелена; Милашин, Јелена; Тољић, Бошко; Николић, Нађа; Парпура, Владимир; Гајић, Радош; Бекyарова, Елена;
dc.citation.volume106
dc.citation.issue10
dc.citation.spage2653
dc.citation.epage2661
dc.citation.other106(10): 2653-2661
dc.citation.rankM22
dc.identifier.wos000448177600009
dc.identifier.doi10.1002/jbm.a.36461
dc.identifier.pmid29896770
dc.identifier.scopus2-s2.0-85052912041
dc.identifier.rcubconv_3566
dc.type.versionpublishedVersion


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