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dc.creatorBaćević, Miljana
dc.creatorBrković, Božidar
dc.creatorLamber, France
dc.creatorĐukić, Ljiljana
dc.creatorPetrović, Nina
dc.creatorRoganović, Jelena
dc.date.accessioned2020-07-02T13:21:58Z
dc.date.available2020-07-02T13:21:58Z
dc.date.issued2019
dc.identifier.issn0003-9969
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/2388
dc.description.abstractObjective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175021/RS//
dc.rightsrestrictedAccess
dc.sourceArchives of Oral Biology
dc.subjectmicroRNA-21en
dc.subjectDiabetesen
dc.subjectL-PRFen
dc.subjectMMP-9en
dc.subjectOxidative stressen
dc.subjectBone heatingen
dc.titleLeukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbitsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЂукић, Љиљана; Рогановић, Јелена; Петровић, Нина; Ламбер, Франце; Брковић, Божидар; Баћевић, Миљана;
dc.citation.volume102
dc.citation.spage231
dc.citation.epage237
dc.citation.other102: 231-237
dc.citation.rankM22
dc.identifier.wos000471206900032
dc.identifier.doi10.1016/j.archoralbio.2019.05.005
dc.identifier.pmid31082699
dc.identifier.scopus2-s2.0-85065195457
dc.type.versionpublishedVersion


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