Приказ основних података о документу

dc.creatorSimonović, Jelena
dc.creatorToljić, Boško
dc.creatorRasković, Bozidar
dc.creatorJovanović, Vladimir
dc.creatorLazarević, Miloš
dc.creatorMilošević, Maja
dc.creatorNikolić, Nadja
dc.creatorPanajotović, Radmila
dc.creatorMilašin, Jelena
dc.date.accessioned2020-07-02T13:22:32Z
dc.date.available2020-07-02T13:22:32Z
dc.date.issued2019
dc.identifier.issn0353-9504
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/2397
dc.description.abstractAim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.en
dc.publisherMedicinska Naklada, Zagreb
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175075/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceCroatian Medical Journal
dc.titleRaman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cellsen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractНиколић, Нађа; Тољић, Бошко; Панајотовић, Радмила; Лазаревић, Милош; Симоновић, Јелена; Јовановић, Владимир; Расковић, Бозидар; Милошевић, Маја; Милашин, Јелена;
dc.citation.volume60
dc.citation.issue2
dc.citation.spage78
dc.citation.epage86
dc.citation.other60(2): 78-86
dc.citation.rankM23
dc.identifier.wos000470656200004
dc.identifier.doi10.3325/CroatMedJ_60_0078
dc.identifier.pmid31044579
dc.identifier.fulltexthttps://smile.stomf.bg.ac.rs/bitstream/id/3435/2392.pdf
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу