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dc.creatorParaš, S.
dc.creatorTrišić, Dijana
dc.creatorAjtić, O.M.
dc.creatorProkić, B.
dc.creatorDrobne, D.
dc.creatorŽivković, Slavoljub
dc.creatorJokanović, Vukoman
dc.date.accessioned2020-07-02T13:27:38Z
dc.date.available2020-07-02T13:27:38Z
dc.date.issued2020
dc.identifier.issn2079-4991
dc.identifier.urihttp://smile.stomf.bg.ac.rs/handle/123456789/2478
dc.description.abstractNovel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.en
dc.publisherMdpi Ag
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172026/RS//
dc.relationSlovenian research agency (J1-9162)
dc.rightsopenAccess
dc.sourceNanomaterials
dc.subjectBiocompatibilityen
dc.subjectBone substituteen
dc.subjectGenotoxicityen
dc.subjectHydroxyapatiteen
dc.subjectSubchronic toxicityen
dc.titleToxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of ratsen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractЈокановић, Вукоман; Дробне, Д.; Живковић, Славољуб; Тришић, Дијана; Прокић, Б.; Параш, С.; Aјтић, О.М.;
dc.citation.volume10
dc.citation.issue5
dc.citation.other10(5): -
dc.citation.rankM21~
dc.identifier.wos000540781800101
dc.identifier.doi10.3390/nano10050918
dc.identifier.scopus2-s2.0-85085069788
dc.identifier.fulltexthttp://smile.stomf.bg.ac.rs/bitstream/id/1002/2473.pdf
dc.identifier.rcubconv_3878
dc.type.versionpublishedVersion


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