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dc.creatorLazarević, Miloš
dc.creatorMilošević, Maja
dc.creatorJelovac, Drago
dc.creatorMilenković, Sanja
dc.creatorTepavčević, Zvezdana
dc.creatorBaldan, Federica
dc.creatorSuboticki, Tijana
dc.creatorToljić, Boško
dc.creatorTrišić, Dijana
dc.creatorDragović, Miroslav
dc.creatorDamante, Giuseppe
dc.creatorMilašin, Jelena
dc.date.accessioned2020-07-02T13:28:00Z
dc.date.available2020-07-02T13:28:00Z
dc.date.issued2020
dc.identifier.issn1792-1074
dc.identifier.urihttps://smile.stomf.bg.ac.rs/handle/123456789/2484
dc.description.abstractEpithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.en
dc.publisherSPANDIDOS PUBL LTD, ATHENS
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175075/RS//
dc.relationMinistry of Foreign Affairs of Italy [PGR02954]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41027/RS//
dc.rightsopenAccess
dc.sourceOncology Letters
dc.subjectoral canceren
dc.subjectepithelial to mesenchymal transitionen
dc.subjectsurgical marginsen
dc.subjectgene expressionen
dc.subjectcell proliferation and migrationen
dc.titleMarked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro studyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractТришић, Дијана; Драговић, Мирослав; Даманте, Гиусеппе; Тепавчевић, Звездана; Миленковић, Сања; Суботицки, Тијана; Лазаревић, Милош; Балдан, Федерица; Тољић, Бошко; Милашин, Јелена; Јеловац, Драго; Милошевић, Маја;
dc.citation.volume19
dc.citation.issue6
dc.citation.spage3743
dc.citation.epage3750
dc.citation.other19(6): 3743-3750
dc.citation.rankM23~
dc.identifier.wos000535780700015
dc.identifier.doi10.3892/ol.2020.11494
dc.identifier.pmid32382327
dc.identifier.scopus2-s2.0-85083633185
dc.identifier.fulltexthttps://smile.stomf.bg.ac.rs/bitstream/id/1005/2479.pdf
dc.type.versionpublishedVersion


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