Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.
TY - JOUR
AU - Šljivančanin Jakovljević, Tamara
AU - Kontić-Vučinić, Olivera
AU - Nikolić, Nadja
AU - Čarkić, Jelena
AU - Milašin, Jelena
PY - 2020
UR - https://smile.stomf.bg.ac.rs/handle/123456789/2572
AB - Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.
PB - Taylor & Francis Inc.
T2 - Hypertension in Pregnancy
T1 - VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia
VL - 39
IS - 4
SP - 471
EP - 480
DO - 10.1080/10641955.2020.1843663
ER -
@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Milašin, Jelena",
year = "2020",
abstract = "Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.",
publisher = "Taylor & Francis Inc.",
journal = "Hypertension in Pregnancy",
title = "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia",
volume = "39",
number = "4",
pages = "471-480",
doi = "10.1080/10641955.2020.1843663"
}
Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J.,& Milašin, J.. (2020). VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy
Taylor & Francis Inc.., 39(4), 471-480.
https://doi.org/10.1080/10641955.2020.1843663
Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Milašin J. VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy. 2020;39(4):471-480.
doi:10.1080/10641955.2020.1843663 .
Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Milašin, Jelena, "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia" in Hypertension in Pregnancy, 39, no. 4 (2020):471-480,
https://doi.org/10.1080/10641955.2020.1843663 . .
