Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential
Аутори
Milošević-Marković, MajaLazarević, Miloš
Toljić, Boško
Petrović, Milan
Vukadinović, Miroslav
Jezdić, Zoran
Aničić, Boban
Jelovac, Drago
Jovanović, Svetlana
Milašin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC).
Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies.
However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of
CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell
morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP,
Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2)
markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high
expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the
levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation... and neuro-differentiation were
unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially
be useful in the development of new strategies for the treatment of aggressive BCCs.
Кључне речи:
Basal cell carcinoma / Cancer stem cells / Differentiation potential / Osteo-differentiation / Chondro-differentiationИзвор:
BIOCELL, 2021, 45, 6, 1543-1550Издавач:
- TECH SCIENCE PRESS
Финансирање / пројекти:
- Ministry of Education, Science and Technological Development, Republic of Serbia [451-03-9/2021-14/200129]
DOI: 10.32604/biocell.2021.015060
ISSN: 0327-9545
WoS: 000692530400013
Scopus: 2-s2.0-85115184816
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Milošević-Marković, Maja AU - Lazarević, Miloš AU - Toljić, Boško AU - Petrović, Milan AU - Vukadinović, Miroslav AU - Jezdić, Zoran AU - Aničić, Boban AU - Jelovac, Drago AU - Jovanović, Svetlana AU - Milašin, Jelena PY - 2021 UR - https://smile.stomf.bg.ac.rs/handle/123456789/3283 AB - Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC). Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies. However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP, Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2) markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation and neuro-differentiation were unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially be useful in the development of new strategies for the treatment of aggressive BCCs. PB - TECH SCIENCE PRESS T2 - BIOCELL T1 - Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential VL - 45 IS - 6 SP - 1543 EP - 1550 DO - 10.32604/biocell.2021.015060 ER -
@article{ author = "Milošević-Marković, Maja and Lazarević, Miloš and Toljić, Boško and Petrović, Milan and Vukadinović, Miroslav and Jezdić, Zoran and Aničić, Boban and Jelovac, Drago and Jovanović, Svetlana and Milašin, Jelena", year = "2021", abstract = "Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC). Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies. However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP, Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2) markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation and neuro-differentiation were unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially be useful in the development of new strategies for the treatment of aggressive BCCs.", publisher = "TECH SCIENCE PRESS", journal = "BIOCELL", title = "Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential", volume = "45", number = "6", pages = "1543-1550", doi = "10.32604/biocell.2021.015060" }
Milošević-Marković, M., Lazarević, M., Toljić, B., Petrović, M., Vukadinović, M., Jezdić, Z., Aničić, B., Jelovac, D., Jovanović, S.,& Milašin, J.. (2021). Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential. in BIOCELL TECH SCIENCE PRESS., 45(6), 1543-1550. https://doi.org/10.32604/biocell.2021.015060
Milošević-Marković M, Lazarević M, Toljić B, Petrović M, Vukadinović M, Jezdić Z, Aničić B, Jelovac D, Jovanović S, Milašin J. Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential. in BIOCELL. 2021;45(6):1543-1550. doi:10.32604/biocell.2021.015060 .
Milošević-Marković, Maja, Lazarević, Miloš, Toljić, Boško, Petrović, Milan, Vukadinović, Miroslav, Jezdić, Zoran, Aničić, Boban, Jelovac, Drago, Jovanović, Svetlana, Milašin, Jelena, "Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential" in BIOCELL, 45, no. 6 (2021):1543-1550, https://doi.org/10.32604/biocell.2021.015060 . .