Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease

Abstract

Periodontal disease is a chronic inflammatory disease caused by a small group of mainly anaerobic gram-negative bacteria, which leads to the destruction of all tissues of periodontium. Although bacterias are essential they are not enough to lead to the development of disease. Recent data indicate the importance of viruses in the pathogenesis of periodontal disease. The infection leads to inflammatory destruction of periodontal tissues. Oral anaerobic microorganisms and their products, such as lipopolysaccharide (LPS) present in subgingival dental plaque, penetrating into the connective tissue of the gingiva due in systemic circulation through ulcerated and discontinuous epithelium of the periodontal pocket. Currently, it is believed that the pathogenesis of some types of periodontal disease process of multiple steps including the complex interaction between the host, bacteria, viruses, and various environmental factors. The starting hypothesis: for the formation of periodontal bacteria, essential, but not sufficient to lead to the development of the disease, herpes viruses, EBV and CMV may provide answers to some open questions related to the mechanism of degradation of periodontal tissues as well as the consequent complications. Objectives: 1. Identifying presence of specific bacterial flora: Porphyromonas gingivalis(Pg), Tannerella forsythensis(Tf), Prevotella intermedi (Pi) and Aggregatibacter actinomycetemcomitans(A.g). 2. Detection of herpes viridae family members: Epstein Barr virus(EBV), human cytomegalovirus(HCMV) and herpes simplex virus type 1(HSV-1) with complications of periodontal disease (periodontitis and necrotizing ulcer-periodontal abscess)...

Parodontopatija je hronično inflamatorno oboljenje uzrokovano malom grupom uglavnom anaerobnih Gram-negativnih bakterija, u kom dolazi do destrukcije svih tkiva parodoncijuma. Iako su bakterije esencijalne one nisu dovoljne da dovedu do razvoja bolesti. Noviji podaci ukazuju na značaj virusa u patogenezi parodontopatije. Infekcija dovodi do inflamatorne destrukcije svih tkiva parodoncijuma. Oralni anaerobni mikroorganizmi i njihovi produkti, kao što je lipopolisaharid (LPS) prisutni u subgingivalnom dentalnom plaku, prodorom u vezivno tkivo gingive dospevaju u sistemsku cirkulaciju kroz ulcerisan i diskontinuiran epitel parodontalnog džepa. Trenutno, veruje se da je patogeneza nekih tipova parodontopatije proces od više koraka, uključujući složenu interakciju između domaćina, bakterija, virusa i raznih faktora okoline. Polazna hipoteza : za nastanak parodontopatije bakterije jesu esencijalne ali ne i dovoljne da dovedu do razvoja bolesti, herpes virusi, EBV i CMV mogu dati odgovore na neka otvorena pitanja vezana za mehanizam degradacije parodontalnih tkiva kao i posledičnih komplikacija. Ciljevi istraživanja : 1. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog bakterijskog genoma (Porphyromonas gingivalis (P.g), Tannerella forsythensis (T.f), Prevotella intermedia (P.i) i Aggregatibacter actinomycetemcomitans (A.g) kod komplikacija parodontopatije (ulcero- nekrozna parodontopatija i parodontalni apsces). 2. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog virusnog genoma Epštajn Barov virus (EBV), Humani Citomegalovirus (HCMV) i herpes simplex virus tip 1 (HSV-1) kod komplikacija parodontopatije (ulceronekrozna parodontopatija i parodontalni apsces)...