Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate
Samo za registrovane korisnike
2012
Autori
Gašić, JovankaPopović, Jelena
Živković, Slavoljub
Petrović, Aleksandar
Barac, Radomir
Nikolić, Marija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p lt 0.001).... Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX. Microsc. Res. Tech. 75:10991103, 2012.
Ključne reči:
endodontics / chlorhexidine gluconate / sodium hypochlorite / interaction / precipitateIzvor:
Microscopy Research & Technique, 2012, 75, 8, 1099-1103Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Antioksidativna zaštita i potencijali za diferencijaciju i regeneraciju mezenhimalnih matičnih ćelija iz različitih tkiva tokom procesa starenja (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1002/jemt.22036
ISSN: 1059-910X
PubMed: 22419366
WoS: 000306786500013
Scopus: 2-s2.0-84864294878
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Gašić, Jovanka AU - Popović, Jelena AU - Živković, Slavoljub AU - Petrović, Aleksandar AU - Barac, Radomir AU - Nikolić, Marija PY - 2012 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1764 AB - To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p lt 0.001). Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX. Microsc. Res. Tech. 75:10991103, 2012. PB - Wiley, Hoboken T2 - Microscopy Research & Technique T1 - Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate VL - 75 IS - 8 SP - 1099 EP - 1103 DO - 10.1002/jemt.22036 ER -
@article{ author = "Gašić, Jovanka and Popović, Jelena and Živković, Slavoljub and Petrović, Aleksandar and Barac, Radomir and Nikolić, Marija", year = "2012", abstract = "To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p lt 0.001). Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX. Microsc. Res. Tech. 75:10991103, 2012.", publisher = "Wiley, Hoboken", journal = "Microscopy Research & Technique", title = "Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate", volume = "75", number = "8", pages = "1099-1103", doi = "10.1002/jemt.22036" }
Gašić, J., Popović, J., Živković, S., Petrović, A., Barac, R.,& Nikolić, M.. (2012). Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate. in Microscopy Research & Technique Wiley, Hoboken., 75(8), 1099-1103. https://doi.org/10.1002/jemt.22036
Gašić J, Popović J, Živković S, Petrović A, Barac R, Nikolić M. Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate. in Microscopy Research & Technique. 2012;75(8):1099-1103. doi:10.1002/jemt.22036 .
Gašić, Jovanka, Popović, Jelena, Živković, Slavoljub, Petrović, Aleksandar, Barac, Radomir, Nikolić, Marija, "Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate" in Microscopy Research & Technique, 75, no. 8 (2012):1099-1103, https://doi.org/10.1002/jemt.22036 . .