Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment
2015
Authors
Puač, NevenaMiletić, Maja
Mojović, Miloš
Popović-Bijelić, Ana
Vuković, Dragana
Miličić, Biljana
Maletić, Dejan
Lazović, Saša
Malović, Gordana
Petrović, Zoran Lj.
Article (Published version)
Metadata
Show full item recordAbstract
In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilizati...on was achieved in the case of gram negative bacteria.
Keywords:
plasma needle / sterilization / spin-trap / Electron Paramagnetic Resonance / radicalsSource:
Open Chemistry, 2015, 13, 1, 332-338Publisher:
- De Gruyter Open Ltd, Warsaw
Funding / projects:
- Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Antibiotic resistant bacterial pathogens in Serbia: phenotypic and genotypic characterization (RS-MESTD-Basic Research (BR or ON)-175039)
- Fundamental processes and applications of particle transport in non-equilibrium plasmas, traps and nanostructures (RS-MESTD-Basic Research (BR or ON)-171037)
DOI: 10.1515/chem-2015-0041
ISSN: 2391-5420
WoS: 000355403100040
Scopus: 2-s2.0-84982719221
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Institution/Community
Stomatološki fakultetTY - JOUR AU - Puač, Nevena AU - Miletić, Maja AU - Mojović, Miloš AU - Popović-Bijelić, Ana AU - Vuković, Dragana AU - Miličić, Biljana AU - Maletić, Dejan AU - Lazović, Saša AU - Malović, Gordana AU - Petrović, Zoran Lj. PY - 2015 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1966 AB - In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria. PB - De Gruyter Open Ltd, Warsaw T2 - Open Chemistry T1 - Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment VL - 13 IS - 1 SP - 332 EP - 338 DO - 10.1515/chem-2015-0041 ER -
@article{ author = "Puač, Nevena and Miletić, Maja and Mojović, Miloš and Popović-Bijelić, Ana and Vuković, Dragana and Miličić, Biljana and Maletić, Dejan and Lazović, Saša and Malović, Gordana and Petrović, Zoran Lj.", year = "2015", abstract = "In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.", publisher = "De Gruyter Open Ltd, Warsaw", journal = "Open Chemistry", title = "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment", volume = "13", number = "1", pages = "332-338", doi = "10.1515/chem-2015-0041" }
Puač, N., Miletić, M., Mojović, M., Popović-Bijelić, A., Vuković, D., Miličić, B., Maletić, D., Lazović, S., Malović, G.,& Petrović, Z. Lj.. (2015). Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry De Gruyter Open Ltd, Warsaw., 13(1), 332-338. https://doi.org/10.1515/chem-2015-0041
Puač N, Miletić M, Mojović M, Popović-Bijelić A, Vuković D, Miličić B, Maletić D, Lazović S, Malović G, Petrović ZL. Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry. 2015;13(1):332-338. doi:10.1515/chem-2015-0041 .
Puač, Nevena, Miletić, Maja, Mojović, Miloš, Popović-Bijelić, Ana, Vuković, Dragana, Miličić, Biljana, Maletić, Dejan, Lazović, Saša, Malović, Gordana, Petrović, Zoran Lj., "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment" in Open Chemistry, 13, no. 1 (2015):332-338, https://doi.org/10.1515/chem-2015-0041 . .