dc.creator | Petrović, Nina | |
dc.creator | Jovanović-Cupić, Snezana | |
dc.creator | Brajušković, Goran | |
dc.creator | Lukić, Silvana | |
dc.creator | Roganović, Jelena | |
dc.creator | Krajnović, Milena | |
dc.creator | Mandusić, Vesna | |
dc.date.accessioned | 2020-07-02T12:57:04Z | |
dc.date.available | 2020-07-02T12:57:04Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | https://smile.stomf.bg.ac.rs/handle/123456789/1991 | |
dc.description.abstract | Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels. | en |
dc.publisher | Srpsko biološko društvo, Beograd, i dr. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173049/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of Biological Sciences | |
dc.subject | Invasive ductal carcinoma with non-invasive component (IDC-DCIS) | en |
dc.subject | miR-21 expression levels | en |
dc.subject | the difference between IDC-DCIS and IDC | en |
dc.title | Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Мандусић, Весна; Рогановић, Јелена; Брајушковић, Горан; Петровић, Нина; Лукић, Силвана; Јовановић-Цупић, Снезана; Крајновић, Милена; | |
dc.citation.volume | 67 | |
dc.citation.issue | 4 | |
dc.citation.spage | 1285 | |
dc.citation.epage | 1295 | |
dc.citation.other | 67(4): 1285-1295 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000366795700020 | |
dc.identifier.doi | 10.2298/ABS150327105P | |
dc.identifier.scopus | 2-s2.0-84951790728 | |
dc.identifier.fulltext | https://smile.stomf.bg.ac.rs/bitstream/id/639/1986.pdf | |
dc.type.version | publishedVersion | |