Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions
Само за регистроване кориснике
2015
Аутори
Jakovljević, AleksandarAndrić, Miroslav
Knežević, Aleksandra
Soldatović, Ivan
Nikolić, Nadja
Karalić, Danijela
Milašin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB typ...e III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.
Кључне речи:
Apical periodontitis / EBNA-2 protein / Epstein-Barr virus / genotype / glycoprotein B / human cytomegalovirusИзвор:
Journal of Endodontics, 2015, 41, 11, 1847-1851Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- Генетичка контрола и молекуларни механизми у малигним, инфламаторним и развојним патологијама орофацијалне регије (RS-MESTD-Basic Research (BR or ON)-175075)
- Медицински значај генетичке варијабилности вируса (RS-MESTD-Basic Research (BR or ON)-175073)
DOI: 10.1016/j.joen.2015.08.027
ISSN: 0099-2399
PubMed: 26435468
WoS: 000364437600011
Scopus: 2-s2.0-84945580007
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Jakovljević, Aleksandar AU - Andrić, Miroslav AU - Knežević, Aleksandra AU - Soldatović, Ivan AU - Nikolić, Nadja AU - Karalić, Danijela AU - Milašin, Jelena PY - 2015 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1996 AB - Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions. PB - Elsevier Science Inc, New York T2 - Journal of Endodontics T1 - Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions VL - 41 IS - 11 SP - 1847 EP - 1851 DO - 10.1016/j.joen.2015.08.027 ER -
@article{ author = "Jakovljević, Aleksandar and Andrić, Miroslav and Knežević, Aleksandra and Soldatović, Ivan and Nikolić, Nadja and Karalić, Danijela and Milašin, Jelena", year = "2015", abstract = "Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Endodontics", title = "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions", volume = "41", number = "11", pages = "1847-1851", doi = "10.1016/j.joen.2015.08.027" }
Jakovljević, A., Andrić, M., Knežević, A., Soldatović, I., Nikolić, N., Karalić, D.,& Milašin, J.. (2015). Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics Elsevier Science Inc, New York., 41(11), 1847-1851. https://doi.org/10.1016/j.joen.2015.08.027
Jakovljević A, Andrić M, Knežević A, Soldatović I, Nikolić N, Karalić D, Milašin J. Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics. 2015;41(11):1847-1851. doi:10.1016/j.joen.2015.08.027 .
Jakovljević, Aleksandar, Andrić, Miroslav, Knežević, Aleksandra, Soldatović, Ivan, Nikolić, Nadja, Karalić, Danijela, Milašin, Jelena, "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions" in Journal of Endodontics, 41, no. 11 (2015):1847-1851, https://doi.org/10.1016/j.joen.2015.08.027 . .