The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2
Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2
Apstrakt
Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in ...capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping.
Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9... pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.
Ključne reči:
dental pulp capping / diabetes / vascular endothelial growth factor / bone morphogenetic protein 2 / calcium hydroxide / prekrivanje pulpe / dijabetes / vaskularni endotelni faktor rasta / kostni morfogenetski protein 2 / kalcijum-hidroksidIzvor:
Srpski arhiv za celokupno lekarstvo, 2020, 148, 11-12, 673-678Izdavač:
- Beograd: Srpsko lekarsko društvo
Finansiranje / projekti:
DOI: 10.2298/SARH200228057I
ISSN: 0370-8179
WoS: 000604439300002
Scopus: 2-s2.0-85099955941
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Ilić, Jugoslav AU - Radović, Katarina AU - Brković, Božidar AU - Vasić, Jugoslav AU - Roganović, Jelena PY - 2020 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2588 AB - Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping. AB - Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe. PB - Beograd: Srpsko lekarsko društvo T2 - Srpski arhiv za celokupno lekarstvo T1 - The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2 T1 - Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2 VL - 148 IS - 11-12 SP - 673 EP - 678 DO - 10.2298/SARH200228057I ER -
@article{ author = "Ilić, Jugoslav and Radović, Katarina and Brković, Božidar and Vasić, Jugoslav and Roganović, Jelena", year = "2020", abstract = "Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping., Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.", publisher = "Beograd: Srpsko lekarsko društvo", journal = "Srpski arhiv za celokupno lekarstvo", title = "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2, Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2", volume = "148", number = "11-12", pages = "673-678", doi = "10.2298/SARH200228057I" }
Ilić, J., Radović, K., Brković, B., Vasić, J.,& Roganović, J.. (2020). The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo Beograd: Srpsko lekarsko društvo., 148(11-12), 673-678. https://doi.org/10.2298/SARH200228057I
Ilić J, Radović K, Brković B, Vasić J, Roganović J. The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo. 2020;148(11-12):673-678. doi:10.2298/SARH200228057I .
Ilić, Jugoslav, Radović, Katarina, Brković, Božidar, Vasić, Jugoslav, Roganović, Jelena, "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2" in Srpski arhiv za celokupno lekarstvo, 148, no. 11-12 (2020):673-678, https://doi.org/10.2298/SARH200228057I . .