The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
Аутори
Simonović, JelenaToljić, Boško
Lazarević, Miloš
Milošević-Marković, Maja
Perić, Mina
Vujin, Jasna
Panajotović, Radmila
Milašin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results:... SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.
Кључне речи:
graphene / dental stem cells / stem cells from apical papilla / neurogenic differentiationИзвор:
Nanomaterials, 2022, 12, 18, 3116-Издавач:
- MDPI
Финансирање / пројекти:
- Ministry of Education, Science and Technological Development of the Republic of Serbia [451-03-9/2021-14/200129]; Science Fund of the Republic of Serbia [7750038]
DOI: 10.3390/nano12183116
ISSN: 2079-4991
PubMed: 36144905
WoS: 000857573800001
Scopus: 2-s2.0-85138608251
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Simonović, Jelena AU - Toljić, Boško AU - Lazarević, Miloš AU - Milošević-Marković, Maja AU - Perić, Mina AU - Vujin, Jasna AU - Panajotović, Radmila AU - Milašin, Jelena PY - 2022 UR - https://smile.stomf.bg.ac.rs/handle/123456789/3282 AB - Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid- phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film. PB - MDPI T2 - Nanomaterials T1 - The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla VL - 12 IS - 18 SP - 3116 DO - 10.3390/nano12183116 ER -
@article{ author = "Simonović, Jelena and Toljić, Boško and Lazarević, Miloš and Milošević-Marković, Maja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Milašin, Jelena", year = "2022", abstract = "Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid- phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film.", publisher = "MDPI", journal = "Nanomaterials", title = "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla", volume = "12", number = "18", pages = "3116", doi = "10.3390/nano12183116" }
Simonović, J., Toljić, B., Lazarević, M., Milošević-Marković, M., Perić, M., Vujin, J., Panajotović, R.,& Milašin, J.. (2022). The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials MDPI., 12(18), 3116. https://doi.org/10.3390/nano12183116
Simonović J, Toljić B, Lazarević M, Milošević-Marković M, Perić M, Vujin J, Panajotović R, Milašin J. The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials. 2022;12(18):3116. doi:10.3390/nano12183116 .
Simonović, Jelena, Toljić, Boško, Lazarević, Miloš, Milošević-Marković, Maja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Milašin, Jelena, "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla" in Nanomaterials, 12, no. 18 (2022):3116, https://doi.org/10.3390/nano12183116 . .