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The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
dc.creator | Simonović, Jelena | |
dc.creator | Toljić, Boško | |
dc.creator | Lazarević, Miloš | |
dc.creator | Milošević-Marković, Maja | |
dc.creator | Perić, Mina | |
dc.creator | Vujin, Jasna | |
dc.creator | Panajotović, Radmila | |
dc.creator | Milašin, Jelena | |
dc.date.accessioned | 2023-12-13T16:40:29Z | |
dc.date.available | 2023-12-13T16:40:29Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2079-4991 | |
dc.identifier.uri | https://smile.stomf.bg.ac.rs/handle/123456789/3282 | |
dc.description.abstract | Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid- phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film. | sr |
dc.language.iso | en | sr |
dc.publisher | MDPI | sr |
dc.relation | Ministry of Education, Science and Technological Development of the Republic of Serbia [451-03-9/2021-14/200129]; Science Fund of the Republic of Serbia [7750038] | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Nanomaterials | sr |
dc.subject | graphene | sr |
dc.subject | dental stem cells | sr |
dc.subject | stem cells from apical papilla | sr |
dc.subject | neurogenic differentiation | sr |
dc.title | The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.citation.volume | 12 | |
dc.citation.issue | 18 | |
dc.citation.spage | 3116 | |
dc.identifier.wos | 000857573800001 | |
dc.identifier.doi | 10.3390/nano12183116 | |
dc.identifier.pmid | 36144905 | |
dc.identifier.scopus | 2-s2.0-85138608251 | |
dc.identifier.fulltext | http://smile.stomf.bg.ac.rs/bitstream/id/8612/nanomaterials-12-03116-1.pdf | |
dc.type.version | publishedVersion | sr |