TY  - JOUR
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Ilic-Dimitrijević, Ivana
AU  - Eljabo, Najib
AU  - Radunović, Milena
AU  - Aničić, Boban
AU  - Tanić, Nasta
AU  - Falk, Markus
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2317
AB  - Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P  lt  .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P  lt  .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.
PB  - Elsevier Science Inc, New York
T2  - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
T1  - P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population
VL  - 125
IS  - 1
SP  - 52
EP  - 58
DO  - 10.1016/j.oooo.2017.09.013
ER  - 

@article{
author = "Nikolić, Nadja and Čarkić, Jelena and Ilic-Dimitrijević, Ivana and Eljabo, Najib and Radunović, Milena and Aničić, Boban and Tanić, Nasta and Falk, Markus and Milašin, Jelena",
year = "2018",
abstract = "Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P  lt  .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P  lt  .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.",
publisher = "Elsevier Science Inc, New York",
journal = "Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology",
title = "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population",
volume = "125",
number = "1",
pages = "52-58",
doi = "10.1016/j.oooo.2017.09.013"
}

Nikolić, N., Čarkić, J., Ilic-Dimitrijević, I., Eljabo, N., Radunović, M., Aničić, B., Tanić, N., Falk, M.,& Milašin, J.. (2018). P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
Elsevier Science Inc, New York., 125(1), 52-58.
https://doi.org/10.1016/j.oooo.2017.09.013

Nikolić N, Čarkić J, Ilic-Dimitrijević I, Eljabo N, Radunović M, Aničić B, Tanić N, Falk M, Milašin J. P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology. 2018;125(1):52-58.
doi:10.1016/j.oooo.2017.09.013 .

Nikolić, Nadja, Čarkić, Jelena, Ilic-Dimitrijević, Ivana, Eljabo, Najib, Radunović, Milena, Aničić, Boban, Tanić, Nasta, Falk, Markus, Milašin, Jelena, "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population" in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 125, no. 1 (2018):52-58,
https://doi.org/10.1016/j.oooo.2017.09.013 . .