Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts
Authorized Users Only
2016
Authors
Velicković, MilenaPejnović, Nada
Petrović, Renata
Mitrović, Slobodanka
Jeftić, Ilija
Kanjevac, Tatjana
Lukić, Aleksandra
Article (Published version)
Metadata
Show full item recordAbstract
BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of ...IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.
Keywords:
inflammation / interleukin-33 / periapical granuloma / radicular cyst / ST2Source:
Journal of Oral Pathology & Medicine, 2016, 45, 1, 70-76Publisher:
- Wiley, Hoboken
Funding / projects:
- Interleukin 33 /ST2 axis and galectin-3 in the pathogenesis of experimental periapical lesions. (RS-MESTD-Basic Research (BR or ON)-175071)
DOI: 10.1111/jop.12312
ISSN: 0904-2512
PubMed: 25677987
WoS: 000369990100012
Scopus: 2-s2.0-84955747679
Collections
Institution/Community
Stomatološki fakultetTY - JOUR AU - Velicković, Milena AU - Pejnović, Nada AU - Petrović, Renata AU - Mitrović, Slobodanka AU - Jeftić, Ilija AU - Kanjevac, Tatjana AU - Lukić, Aleksandra PY - 2016 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2135 AB - BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis. PB - Wiley, Hoboken T2 - Journal of Oral Pathology & Medicine T1 - Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts VL - 45 IS - 1 SP - 70 EP - 76 DO - 10.1111/jop.12312 ER -
@article{ author = "Velicković, Milena and Pejnović, Nada and Petrović, Renata and Mitrović, Slobodanka and Jeftić, Ilija and Kanjevac, Tatjana and Lukić, Aleksandra", year = "2016", abstract = "BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.", publisher = "Wiley, Hoboken", journal = "Journal of Oral Pathology & Medicine", title = "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts", volume = "45", number = "1", pages = "70-76", doi = "10.1111/jop.12312" }
Velicković, M., Pejnović, N., Petrović, R., Mitrović, S., Jeftić, I., Kanjevac, T.,& Lukić, A.. (2016). Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine Wiley, Hoboken., 45(1), 70-76. https://doi.org/10.1111/jop.12312
Velicković M, Pejnović N, Petrović R, Mitrović S, Jeftić I, Kanjevac T, Lukić A. Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine. 2016;45(1):70-76. doi:10.1111/jop.12312 .
Velicković, Milena, Pejnović, Nada, Petrović, Renata, Mitrović, Slobodanka, Jeftić, Ilija, Kanjevac, Tatjana, Lukić, Aleksandra, "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts" in Journal of Oral Pathology & Medicine, 45, no. 1 (2016):70-76, https://doi.org/10.1111/jop.12312 . .